T辅助细胞因子在特发性泪腺炎小鼠MRL／lpr中的表达研究  

Relative of Th cytokines in MRL/lpr mice--a model for idiopathic dacryoadenitis

作　　者：郭洁[1] 钱江[1] 张锐[1] 袁一飞[1] 

机构地区：[1]复旦大学附属眼耳鼻喉医院跟科,上海200031

出　　处：《中华微生物学和免疫学杂志》2009年第9期782-785,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的利用MRL／Ipr小鼠建立特发性泪腺炎模型，观察其泪腺及颌下淋巴结中IL-4、IL-17、IFN-γ的表达，从而对Th细胞因子在该病中的免疫作用进行探讨。方法观察3月龄MRL／lpr小鼠泪腺淋巴病变并以BALB／c小鼠为正常对照。分别对两组小鼠的泪腺及颌下淋巴结行IL-4、IL-17、IFN-γ免疫组化染色，计算淋巴细胞阳性表达量并对结果进行统计学分析，同时对MRL／lpr小鼠颌下淋巴结行IL-4、IL-17、IFN-γ与CIM荧光双标染色观察。结果MRL／lpr小鼠泪腺浸润淋巴细胞中以IL-4表达为主，IL-17及IFN-γ仅少量表达，三者间差异有统计学意义（P〈0．05）。MRL／lpr小鼠颌下淋巴结中IL-4、IL-17阳性细胞数明显多于IFN-γ阳性细胞（P〈0．05），且IL-4、IL-17、IFN-γ表达均较BALB／c小鼠明显增高（P〈0．05）。MRL／lpr小鼠颌下淋巴结中IL4、IL-17、IFN-γ与CD4细胞存在共表达。结论MRL／lpr小鼠是一种理想的特发性泪腺炎动物模型，IL-4介导的Th2免疫应答在病变中起到主导作用，IL-17与该病发生有关并可能在局部及全身发挥不同的免疫调节作用。Objective To determine the distribution of IL-4, IL-17, IFN-γ in the lacrimal gland lesions and submaxillary lymph nodes of MRL/Ipr mice--a model for idiopathic dacryoadenitis, and investigate the possible role of T-helper cells in the disease. Methods Frozen sections of lacrimal glands and submaxillary lymph nodes from MRL/lpr and BALB/c mice age 3 months were stained with antibodies to the cytokines IL-4, IL-17 and IFN-γ, then calculated the numbers or proportions of positive cells respectively, double staining for CD4 and each cytokine was taken in lymph nodes of MRL/lpr mice additionally. Results The major cytoklne in lacrimal gland lesions is IL-4, which contrasts to little IL-17 or IFN-γ stained （P 〈 0.05 ） , the numbers of cells staining for each cytokine in submaxillary lymph nodes elevate in MRL/lpr mice, and the expressions of IL-4 and IL-17 are greater than IFN-γ significantly. Conclusion Th2 may play an important role in the development of local dacryoadenitis and systemic abnormality in MRL/lpr mice, the role of IL-17 maybe different in lacrimal gland lesions and lymph nodes which need more research.

关 键 词：特发性泪腺炎 MRL/LPR IL-4 IL-17 IFN-Γ 

分 类 号：R777[医药卫生—眼科]