牵张成骨术整复猕猴腭裂骨桥蛋白与骨钙蛋白表达研究  

Expression of osteopotin and osteocalcin during distraction osteogenesis on rhesus with cleft palate

作　　者：刘延山[1] 陈刚[1] 刘毅[1] 李蕊[1] 王之奇[1] 申岱[1] 

机构地区：[1]天津医科大学口腔医院,天津300070

出　　处：《中华整形外科杂志》2009年第5期365-368,共4页Chinese Journal of Plastic Surgery

基　　金：国家自然科学基金资助项目（30500533）

摘　　要：目的以牵张成骨术整复猕猴腭裂骨缺损，定量分析新骨在不同时期骨桥蛋白（osteopotin，OPN）与骨钙蛋白（osteocalcin，OC）的表达水平，探讨新骨生成与改建的规律。方法以猕猴为对象建立腭裂动物模型。实验组动物21只行牵张成骨术整复其腭部软硬组织缺损，关闭裂隙后固定。固定期第1、2、4、6、8、12及24周分别取材，各3只动物。采用实时定量PCR法（real—time，RT-PCR）定量比较OPN与OC的mRNA表达水平，并以酶联免疫吸附试验法（ELISA）定量分析其OPN与OC含量，与实验对照组及健康对照组（各2只动物）结果进行比较。结果固定期第2周OPN mRNA表达上调，第4周达最高（7．59±0．37）；而OC mRNA表达则自第4周开始上调（4．98±0．21），第6周时达最大值（7．94±0．31）；随后开始下降，至第24周时两者的mRNA表达水平接近健康对照组（P〉0．05）。ELISA结果显示：固定期第4、6周OPN分别为（4．75±0．15）ns／mg和（4．86±0．09）ng／mg，OC分别为（3．18±0．16）ng／mg和（3．63±0．33）ng／mg，两者均为高水平表达。至第8～12周以后蛋白表达趋势与其对应mRNA表达基本一致。结论应用牵张成骨术整复腭裂骨缺损，其牵张区域新骨生成，裂隙被骨运送盘移动封闭，腭部裂隙被完全修复。Objective To study the mechanism of new bone formation and remodeling of distraction osteogeuesis（DO） by analysis of the expression of osteopotin（OPN）and osteocalcin（OC）. Methods Rhesus were operated to reconstruct the animal model of cleft palate（ CP）. The CP was closed by DO in experimental group（ n = 21）. After consolidation of 1, 2, 4, 6, 8, 12, 24 weeks, every 3 animals were killed to collect the specimens, respectively. The OPN and OC and their mRNA were detected quantitatively by Real-time RT-PCR and ELISA, respectively. The animals in control group（ n = 2） and sham group（ n = 2） were used as control. Results The mRNA expression of OPN increased since 2nd week of consolidation and reached the peak at 4th week（7.59 ± 0.37）. The mRNA expression of OC was up-regulaed since 4th week, and reach the peak at 6th week（7.94 ± 0.31）. Then they decreased to about the level in sham group at 24th week（ P 〉 0.05）. The OPN and OC were highly expressed during 4 to 6 weeks of consolidation. During 8 to 12 weeks, they decreased like their mRNA expression. Conclusion The intramembraneous new bone formation after DO can reconstruct the bone defect of CP. The new formed bone can be remodeled to be quite normal bone tissue.

关 键 词：骨生成 牵张 腭裂 骨桥蛋白 骨钙蛋白 

分 类 号：R782.2[医药卫生—口腔医学]