莨山唇柱苣苔组织培养与植株再生  

Tissue Culture and Plant Regeneration in Chirita langshanica

作　　者：谭晓风[1] 邓建军[1] 胡孝义[1] 乌云塔娜[1] 包梅荣[1] 

机构地区：[1]中南林业科技大学经济林育种与栽培国家林业局重点实验室,湖南长沙410004

出　　处：《经济林研究》2009年第3期24-27,共4页Non-wood Forest Research

摘　　要：为了能快速繁殖莨山唇柱苣苔,以其嫩叶为试验材料,进行了在不同培养条件下的组织培养研究。研究结果表明:MS+BA 1.0 mg.L-1+NAA 0.5 mg.L-1为愈伤组织诱导的最佳培养基;MS+BA 0.5mg.L-1+NAA 0.3 mg.L-1为丛生芽分化的最佳培养基;MS+NAA 0.3 mg.L-1+石英砂为最佳生根培养基。在80%遮阳网下进行炼苗,移栽25 d后成活率可达80%。In order to realize rapid propagation in Chirita langshanica,taking tender leaves as experimental materials,tissue culture techniques in Chirita langshanica were studied under different culture conditions.The results indicated that MS supplemented with BA 1.0 mg·L-1 and NAA 0.5 mg·L-1 was the optimal medium for inducing callus,MS supplemented with BA 0.5 mg·L-1 and NAA 0.3 mg·L-1 was the best medium for cluster buds differentiation,and MS supplemented with NAA 0.3 mg·L-1 and quartz sand was the optimal medium for inducing root. Hardening seedlings should be carried out under 80% shading-net, and the survival rate of seedlings replanted was up to 800/00 after 25 days.

关 键 词：生物工程 莨山唇柱苣苔 组织培养 植株再生 

分 类 号：S722.37[农业科学—林木遗传育种]