石榴疏松愈伤组织诱导条件的优化  

Optimization of Induction Conditions of Loose Callus from Punica granatum L.

作　　者：程江华[1] 任琪[1] 李宝华 寻华凤[1] 丁之恩[1] 

机构地区：[1]安徽农业大学,安徽合肥230000 [2]江苏泗阳县农林局,江苏泗阳223700

出　　处：《经济林研究》2009年第3期75-78,共4页Non-wood Forest Research

基　　金：国家自然基金项目部分内容(项目编号:30571520)

摘　　要：为给石榴细胞的液体悬浮培养,工厂化生产次生代谢产物提供理论基础,研究了不同外植体、不同灭菌条件、不同激素水平对石榴愈伤组织诱导率、疏松程度及褐化率的影响。结果表明:成熟叶片更容易诱导出疏松愈伤组织,体积分数为75%酒精和质量分数为0.1%的升汞组合的消毒效果更好。石榴愈伤组织的最佳诱导培养基为:MS+1.0 mg.L-16-BA+1.0 mg.L-1NAA。石榴最佳继代培养基为:MS+1.0 mg.L-16-BA+1.0 mg.L-1NAA+1.0 mg.L-12,4-D。In order to provide theoretical basis for liquid suspension culture of cell in Punica Granatum L. and industrialized production of secondary metabolites, effects of different explants, sterilization conditions and hormone concentrations on induction rate, loose degree and browning rate of callus were studied. The results showed that loose callus from mature leave was induced easily, and 75% ethanol combined with 0.1% mercuric chloride had the best disinfection effect. The optimal medium for inducting callus was MS supplemented with 1. 0 mg · L-1 6-BA and 1.0 mg · L-1NAA. The optimal subculture medium was MS supplemented with 1.0 mg · L-1 6-BA, 1.0 mg ·L-1NAA and 1.0 mg· L-1 2,4-D.

关 键 词：生物工程 石榴 疏松愈伤组织 诱导 继代培养 

分 类 号：S665.4[农业科学—果树学]