深层培养云芝菌丝体蛋白多糖的提取及性质  

Extraction and character of proteoglycan from the mycelium of Polystictus versicolor (L.) Fr. by submerged culture\

作　　者：程光宇[1] 吴国荣[1] 邹玉珍[1] 陈胜兰[1] 

机构地区：[1]南京师范大学生物系

出　　处：《植物资源与环境》1998年第4期19-23,共5页Journal of Plant Resources and Environment

摘　　要：采用水、３％草酸和０．２ｍｏｌ／Ｌ氢氧化钠为溶剂分别提取深层培养的云芝〔Ｐｏｌｙｓｔｉｃｔｕｓｖｅｒｓｉｃｏｌｏｒ（Ｌ．）Ｆｒ．〕菌丝体蛋白多糖，其得率分别为菌丝体干重的２０％、４９％和１９％。提取的粗多糖经ＳｅｐｈａｄｅｘＧ１００柱层析进一步分离、纯化，纯化的多糖经聚丙烯酰胺凝胶电泳分离后呈现一个斑点，在ＳｅｐｈａｄｅｘＧ１００柱层析中有一个对称峰，紫外及红外光谱分析和蛋白质含量测定表明该多糖为蛋白多糖，其蛋白质和多糖含量在水提多糖中分别为１３％和６２％、在酸提多糖中分别为５％和９１％。在碱提多糖中分别为３％和８１％。组成该多糖的主要单糖是葡萄糖和木糖。Proteoglycan were extracted by water, 3% oxalic acid and 0.2 mol/L NaOH from the mycelium of Polystictus versicolor (L.) Fr. under submerged culture respectively, and extraction yield of proteoglycan account for mycelium dry weight was of 2.0% (water extraction), 4.9% (acid extraction) and 1.9% (alkali extraction). Proteoglycan was further isolated and purified by Sephadex G 100. Its homogeneity was confirmed by chromatography on Sephadex G 100 and PAGE. By means of IR, UV spectrum and protein content analysis that the polysaccharide belongs to proteoglycan were determined. Its sugar and protein content were up 62% and 13% (water extraction), 91% and 5% (acid extraction), 81% and 3% (alkali extraction) respectively. PC and TLC analysis of proteoglycan hydrolate showed that those sugars were mainly composed of glucose and xylose.

关 键 词：云芝 深层培养 蛋白多糖 提取 

分 类 号：S567.390.4[农业科学—中草药栽培]