马铃薯晚疫病菌基因文库的构建  

CONSTRUCTION OF GENOMIC LIBRARY OF PHYTOPHTHORA INFESTANS DE BARY

作　　者：刘晓鹏 姜宁[2] 何礼远[2] 王克[3] 

机构地区：[1]中国南方马铃薯中心 [2]中国农科院 [3]沈阳农业大学

出　　处：《东北农业大学学报》1998年第3期228-235,共8页Journal of Northeast Agricultural University

基　　金：植物病虫害国家重点实验室开放课题

摘　　要：用酶一氯化苄法制备高分子量晚疫病菌总ＤＮＡ，通过Ｓａｕ３Ａ部分酶切并纯化后得到１５－２５ｋｂ的酶切片段，并以具有广寄主范围的柯斯质粒ＰＬＡ２９１７为载体，用Ｔ４ＤＮＡ连接酶连成为重组ＤＮＡ，包装到入噬菌体颗粒中，转染受体菌Ｅ．ｃｏｌｉＳ１７－１。利用四环素和卡那霉素抗性来筛选鉴定重组子，得到１０６５６个重组子，这些重组子群即为晚疫病菌的基因组文库。Hight molecular weight Phytophthora infestansDNA was prepared by enzymes benzyl chloride method. Following partial digestion with sau3A, DNA fragments ranging from 15 to 25 Kb were selected. The recovered DNA was Ligated to the PLA2917 vectors and packaged. The resulting recombinant DNA was transfered into E. coli S17-1, and 10656 transformants were obtained which constituted the genomic library of the phytophthora infestansde Bary.

关 键 词：马铃薯 晚疫病菌 酶一氯化苄法 PLA2917载体 

分 类 号：S435.32[农业科学—农业昆虫与害虫防治]