解钾菌的筛选  

Separation and Purification of the Potassium-releasing Bacteria

作　　者：周毅峰[1] 罗云霞[1] 刘华中[1] 

机构地区：[1]湖北民族学院生物科学与技术学院,湖北恩施445000

出　　处：《湖北民族学院学报（自然科学版）》2009年第3期285-288,共4页Journal of Hubei Minzu University(Natural Science Edition)

基　　金：生物资源保护与利用湖北省重点实验室(湖北民族学院)开放基金项目(200725);湖北民族学院生物科学与技术学院大学生科研训练计划项目

摘　　要：以钾长石粉为惟一钾源的硅酸盐细菌选择性培养基筛选能够降解土壤中钾元素的细菌.利用梯度稀释分离法和平板划线分离法对其中的细菌进行筛选,用火焰分光光度法测定培养液和细菌细胞内的钾含量,以筛选出高效菌株.结果表明:dk8、dk7、dk14在解钾培养基上生长状况良好,以dk8分解钾长石的能力最强,可作为制作微生物肥料备选用菌.In this paper,we separated and purificated the bacteria for releasing insoluble potassium from soil by the selective medium in which potash feldspar power was treated as the only source of potassium.We diluted the bacteria with gradient and lined on culture plate for separation and purification of the bacteria for release potassium,and used flame spectrophotometer for tests potassium content in nutrient solution and the cell of bacteria,and then selscted high-efficiency bacteria.The results show that dk7,dk8 and dk14 strain can grow well on the selective plate,dk8 is the best one for release available potassium from potash feldspar power.Therefore,dk8 can be used as candidate strain for production of microbial fertilizer.

关 键 词：解钾菌 钾长石 速效钾 

分 类 号：Q939.9[生物学—微生物学]