一株聚磷菌GP44的筛选、鉴定及其聚磷特性研究  

Screening and Identification of Phosphate Accumulating Bacterial Strain GP44 and Its Characterization

作　　者：赵海泉[1] 胡子全[1] 

机构地区：[1]安徽农业大学生命科学学院,合肥230036

出　　处：《土壤》2009年第5期757-763,共7页Soils

基　　金：安徽省教育厅自然基金项目(2006KJ214B)资助

摘　　要：采用纯培养结合蓝白斑筛选法从巢湖和南淝河底泥中分离筛选出能聚磷(P)的11株解P细菌,好氧培养时菌体吸P能力测定结果表明,GP44的菌体含P量达到11.92%,具有较高的聚P能力,对其初步鉴定为鉴定菌株GP44属肠杆菌科中的克雷伯氏菌属土生克雷伯氏菌(K.terrigena)。GP44在废水合成培养基上最佳聚P温度30℃、初始pH为7.5、最佳装液量为120ml/250ml和最适C源是葡萄糖,Mg2+、K+和Fe3+有利于菌株GP44的生物除P。Eleven kinds of bacteria with high capability of accumulating poly-P were isolated and screened from the bottom muds of Chaohu Lake and South Feihe River by using the pure culture and blue and white colored screening methods. Their phosphate accumulating capacities were investigated aerobically in synthesized sewage medium. The results showed that the total phosphorus in the GP44 cells was 11.92%, indicating a higher level of phosphorus accumulation, and it was identified as K.terrigena. GP44 grew in synthesized sewage medium with the optimal phosphate accumulating temperature, initial pH value, aeration ration and carbon source was 30℃, 7.5, 120 ml/250 ml and glucose respectively, and Mg2＋, K＋ and Fe3＋ could promote GP44 to accumulate phosphorus.

关 键 词：聚磷菌 筛选 鉴定 聚磷特性 

分 类 号：X172[环境科学与工程—环境科学]