雌激素对大鼠脑组织缺血-再灌注损伤的保护作用  

Protective effects of estrogen on brain tissue ischemia-reperfusion injury of rats

作　　者：王德伟[1] 朱红[1] 孙嘉斌[1] 

机构地区：[1]潍坊医学院,山东潍坊261042

出　　处：《中国医药导报》2009年第30期17-18,共2页China Medical Herald

摘　　要：目的:研究雌激素对绝经后雌性大鼠局灶性脑缺血-再灌注损伤后NF-κB基因表达和TNF-α含量的影响。方法:采用线栓法复制局灶性脑缺血-再灌注模型,应用RT-PCR法检测NF-κB基因的表达,利用放免法检测TNF-α含量。结果:与模型组大鼠比较,雌激素组脑缺血-再灌注后NF-κB表达明显减弱(P<0.01),TNF-α含量明显减少(P<0.01)。结论:雌激素能抑制大鼠脑缺血-再灌注后NF-κB表达,降低TNF-α含量,这可能是其脑保护作用的机制之一。Objective： To study the effects of estrogen on the expression of NF-KB and TNF-α in ischemia-reperfusion brain tissue of rats. Methods： Middle cerebral artery ischemia-reperfusion rats models were performed by modified fila- ment method. RT-PCR was used to detect the expression of NF-KB. Radioimmunity was used to examine the content of TNF-α. Results： Compared with the model group, the expression of NF-KB remarkably attenuated （P〈0.01）, and the con- tent of TNF-α decreased significantly in the estrogen group （P〈0.01）. Conclusion： Estrogen inhibits the expression of NF- KB, decreases the content of TNF-α after cerebral ischemia-reperfusion. This may be one of the mechanisms in which es- trogen performs its neuro-protection effect.

关 键 词：雌激素 脑缺血-再灌注 NF-ΚB TNF-Α 

分 类 号：R743.31[医药卫生—神经病学与精神病学]