牛耳枫提取物对甜菜夜蛾酚氧化酶的抑制作用  

Inhibitory Effects of Two Compounds Extracted from Daphniphyllum calycinum on Phenoloxidase of Spodotera exigua

作　　者：刘伟[1] 肖婷[2] 杜磊[1] 薛超彬[1] 杨帆[1] 罗万春[1] 

机构地区：[1]山东农业大学植物保护学院/农药毒理与应用技术省级重点实验室,山东泰安271018 [2]江苏丘陵地区镇江农业科学研究所,江苏句容212400

出　　处：《中国农业科学》2009年第10期3720-3725,共6页Scientia Agricultura Sinica

基　　金：国家自然科学基金项目(30571237);教育部博士学科点专项科研基金(20070434006)

摘　　要：【目的】筛选对甜菜夜蛾(Spodoptera exigua(Hübner))酚氧化酶具有高抑制活性的化合物,寻找新型害虫控制剂的线索。【方法】以牛耳枫(Daphniphyllum calycinum Benth.)提取物Deoxycalyciphylline B和Methyl homosecodaphniphyllate为抑制剂,采用酶标仪微量法研究了其对甜菜夜蛾酚氧化酶的抑制活性及抑制类型。【结果】Deoxycalyciphylline B和Methyl homosecodaphniphyllate对甜菜夜蛾酚氧化酶的抑制中浓度(IC50)分别为2.439mmol·L-1和0.879mmol·L-1,2种化合物均为典型的可逆竞争型抑制剂,抑制常数(Ki)分别为2.051mmol·L-1和1.269mmol·L-1。【结论】2种生物碱对甜菜夜蛾酚氧化酶具有较好的抑制活性,可以以这2种化合物或其类似物为模板指导酶抑制剂的分子设计,是寻找新型害虫控制剂的重要途径。【Objective】 The article studies phenoloxidase inhibitors against insect and finds the clues on new target for pest control. 【Method】 The effects of deoxycalyciphylline B and Methyl homosecodaphniphyllate extracted from Daphniphyllum calycinum. on phenoloxidase （PO） of Spodoptera exigua （Hbner） were investigated adopting the microtitrationassay. 【Result】 The results indicated that the two compounds showed different inhibitory effects on the PO and the inhibitor concentrations leading to 50% （IC50） activity lost were estimated to be 2.439 mmol·L^-1 for deoxycalyciphylline B and 0.879 mmol·L^-1 for methyl homosecodaphniphyllate,respectively. The two compounds were also found to be reversible competitive inhibitors and the Ki values were determined to be 2.051 mmol·L^-1 and 1.269 mmol·L^-1,respectively. 【Conclusion】 Two alkaloids have better inhibitory activities on the PO. The two compounds or their analogues can be used as the templates to guide the molecular design of enzyme inhibitors. These are important ways looking for new pest control agents.

关 键 词：牛耳枫 甜菜夜蛾 酚氧化酶 抑制动力学 

分 类 号：S482.3[农业科学—农药学]