激光诱导荧光技术在体探测裸鼠腹膜胃癌播散  

Detection of Gastric Cancer Peritoneal Dissemination in Nude Mouse Model by Laser-Induced Fluorescence Spectroscopy in Vivo

作　　者：马君[1] 张奚宁[1] 徐明[1] 毛伟征[2] 郑荣儿[1] 

机构地区：[1]中国海洋大学光学光电子实验室,山东青岛266100 [2]青岛大学医学院附属医院普外科,山东青岛266003

出　　处：《中国激光》2009年第10期2566-2570,共5页Chinese Journal of Lasers

基　　金：山东省自然科学基金(Y2006G23);青岛市科技发展计划(08-1-3-31-jch)资助课题

摘　　要：以腹膜接种人胃癌细胞SGC-7901的裸鼠为胃癌腹膜播散的动物模型,模拟外科手术,在体探测不同种植期的裸鼠腹膜癌结节及正常腹膜组织的激光诱导自体荧光光谱,比对各组光谱形态,采用层次聚类分析和判别分析方法进行分类。结果表明,还原型烟酰胺腺嘌呤二核苷酸(NADH)荧光峰强度随肿瘤细胞生长时间的增加而降低,而原卟啉IX峰逐渐升高;当类间距离的测度方法选择为欧氏距离平方,分类数取3时,可有效区分正常组、肿瘤细胞种植时间较长和较短的肿瘤组的在体荧光光谱。激光诱导自体荧光(LIAF)技术可辅助提高手术中转移癌的判断准确性。The nude mice which were injected with the human gastric cancer cells （SGC-7901） in their peritoneums were chosen as the animal models of gastric cancer peritoneal dissemination in our research. The laser-induced autofluorescence spectra of these mice which were in different tumor planting periods and the normal counterpart were taken in vivo during the simulated surgeries. The spectra of different tissue types were compared and classified by clustering analysis and discriminate analysis. The results indicate the intensities of reduced nicotinamide adenine dinucleotide （NADH） are gradually decreasing while the one of Protoporphyrin IX （PpIX） are increasing with the growing of the cancer cell. The distinguish of the normal group and the tumor groups with different growth times of cancer cells is quite obvious when the measure method of interclusters and cluster number were set to square euclidean distance and 3,respectively. The technology of laser-induced autofluores cence （LIAF） may help to increase the accuracy of distinguishing the carcinoma metastaticum during surgeries.

关 键 词：激光光谱 胃癌播散 激光诱导自体荧光 裸鼠 聚类分析 判别分析 

分 类 号：Q631[生物学—生物物理学] R735.2[医药卫生—肿瘤]