外周T细胞淋巴瘤TCR基因重排及CDR3谱型分析  

Analyses of the TCR β-chain gene rearrangement and CDR3 repertoire in patients with peripheral T-cell lymphoma

作　　者：张建波[1] 宋永平[2] 于庆凯[1] 白睿华[1] 宋魏[1] 胡俊[3] 董涛[3] 

机构地区：[1]河南省肿瘤医院病理科,郑州450003 [2]河南省肿瘤医院血液科,郑州450003 [3]中山大学中山医学院微生物学教研室

出　　处：《肿瘤研究与临床》2009年第10期657-659,共3页Cancer Research and Clinic

摘　　要：目的分析外周T细胞淋巴瘤穿刺标本中T细胞受体（TCR）β链克隆性基因重排及互补决定区3（CDR3）谱型。方法应用RT—PCR扩增TCR Vβ24个亚家族的CDR3基因，并经基因扫描确定T细胞克隆性，对提示单克隆或寡克隆增生亚家族的PCR产物测序分析其CDR3序列。结果4例淋巴瘤患者TCR表达受到明显抑制，仅表达1～4个Vβ亚家族。所有患者均存在1个或多个Vβ亚家族的克隆增生T细胞，增生形式包括单克隆、双克隆及寡克隆增生趋势。CDR3区序列分析证实增生的T细胞克隆具有不同的氨基酸序列。结论外周T细胞淋巴瘤患者存在T细胞克隆性增生，其TCR Vβ呈限制性取用，不同克隆T细胞具有不同的CDR3谱型。Objective To analyze the clonal gene rearrangement and complementarity determining region 3 （CDR3） Repertoire of TCR IS-chain in fine needle aspiration biopsy （FNAB） specimens of peripheral T-cell lymphoma. Methods The TCR CDR3 region genes of 24 TCR Vβ subfamilies were amplified by utilizing RT-PCR technology, and the CDR3 size lengths of TCR β-chain were analyzed with genesean technology for 4 healthy individuals and 4 patients with peripheral T-cell lymphoma. The clonality of T cells presumed by spectratyping was further confirmed by CDR3 sequencing. Results TCR β-chain presented specific repertoire skewing in 4 cases with peripheral T-cell lymphoma, and only 1-4 TCR Vβ subfamily T cells were identified, respectively. Clonal expanded T cells, including mono, bioclonal and oligoclonal trend patterns, in one or more Vβ subfamilies were found in all cases. The mono expanded T cells have different CDR3 amino acid sequences. Conclusion Characteristic T cells cloning proliferation and selected usage of TCR Vβ subfamily T cells were found in 4 cases with peripheral T-cell lymphoma. The sequences of CDR3 in different TCR clone proliferation are different.

关 键 词：基因重排 互补决定区 淋巴瘤 T细胞 基因扫描 

分 类 号：R733.1[医药卫生—肿瘤]