5种大黄蒽醌类衍生物的同时测定及应用  

Simultaneous Determination and Application of Five Anthraquinone Derivatives of Rhubarb

作　　者：吕慧英[1] 赵晨曦[2] 梁逸曾[1] 吴海[1] 

机构地区：[1]中南大学化学化工学院,中药现代化研究中心,湖南长沙410083 [2]长沙大学生物工程与环境科学系,湖南长沙410003

出　　处：《时珍国医国药》2009年第10期2490-2492,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家科技部中药国际合作项目(No.2007DFA40680);湖南省科技计划项目(No.2007FJ3094)

摘　　要：目的建立同时测定大黄药材中5种蒽醌类衍生物（芦荟大黄素、大黄酸、大黄素、大黄酚和大黄素甲醚）含量的方法,优化大黄蒽醌的超声提取工艺。方法采用反相高效液相法,色谱柱：W aters C18柱（250 mm×4.6 mm,5μm）;流动相：甲醇-乙腈-0.01%磷酸溶液（3∶5∶2）;柱温：25℃;流速：1.0 m l/m in;检测波长：225 nm。结果5种游离蒽醌均具有较宽的线性范围且呈良好的线性关系（R2=0.998 7～0.999 7）,平均回收率为92.79%～99.28%,日内精密度为0.65%～1.71%,日间精密度为2.52%～3.46%。结论该方法成功应用于大黄活性物质的提取工艺优化,根据所建立的方法测得的5种游离蒽醌含量,确定了大黄蒽醌的最佳超声提取工艺为：以70%乙醇为溶剂、料液比为1∶30（g∶m l）、超声提取2次、每次提取20 m in。Objective To develop a method for the simultaneous determination of five anthraquinone derivatives（aloe-emodin,rhein,emodin,chrysophanol and physcion） in rhubarb,and to optimize the ultrasonic extraction conditions.Methods RP-HPLC on a Waters C18 column（250 mm×4.6 mm,5 μm） was used.The mobile phase was methanol-acetonitrile-0.01% phosphoric acid solution（3：5：2）,at a flow rate of 1.0 ml/min,and the detection wavelength was 225 nm.Results The linear ranges of the five free anthraquinones were wide,showing good linear correlations with correlation coefficients（R2） of 0.9987 to 0.9997.The average recoveries were from 92.79% to 99.28%.While the intra-day and inter-day precision were 0.65%1.71% and 2.52%3.46%,respectively.Conclusion Through the contents of five anthraquinone derivatives obtained by the developed RP-HPLC method,the optimum ultrasonic extraction conditions are determined as follows： using 70% EtOH as extracting solvent,the sample is extracted two times and each time for 20 min by a ultrasonic processor,when the solid to liquid ratio is 1：30（g/ml）.

关 键 词：超声提取 大黄 RP-HPLC 蒽醌 

分 类 号：R285.6[医药卫生—中药学]