绿豆皮中总黄酮的提取工艺研究  

Extraction Conditions for Flavonoids from Mung Bean Coat

作　　者：张燕[1] 么杨[2] 潘国清[3] 任贵兴[2] 白根本[1] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]中国农业科学院作物科学研究所,北京100081 [3]中国农业科学院特产研究所,吉林132109

出　　处：《中国粮油学报》2009年第10期124-127,共4页Journal of the Chinese Cereals and Oils Association

基　　金：北京市农业技术试验示范项目(20080807);中国农业科学院人才基金项目(2060302-14)

摘　　要：利用正交试验方法优化得到绿豆皮中总黄酮的最佳提取工艺。以芦丁为标准品,采用分光光度法在510 nm下对提取液中总黄酮含量进行测定。通过提取时间、乙醇体积分数、提取温度、固液比与提取次数5个因素的单因素试验,设计L16(45)正交试验筛选最佳工艺。结果表明:提取时间150 m in,乙醇体积分数50%,提取温度80℃,固液比1∶10,提取次数2次,绿豆皮中总黄酮的提取量为3.879 mg/g,平均回收率为100.84%,精密度试验RSD为0.18%。On the basis of single factor experiment,five influencing factors,namely extracting time,ethanol concentration,extraction temperature,material-liquid ratio and extraction times for extracting flavonoids from mung bean coat were established.Thus a L16（45） orthogonal experiment was applied to optimize the extraction conditions,using spectrophotometry to determine the total flavonoids at 510nm wavelength.Results： The obtained optimal extraction conditions are extracting time 150 min,ethanol concentration 50%, extraction temperature 80℃, material - liquid ratio 1：10 and extraction times 2 times. Under the conditions the extraction rate of flavonoids is 3. 879 mg/g, the average recovery is 100. 84% and RSD is 0. 18%.

关 键 词：绿豆皮 总黄酮 正交试验 分光光度法 

分 类 号：S522[农业科学—作物学]