转导野生型p53基因提高K562细胞对紫外线诱导的细胞凋亡的敏感性  

Enhancement of apoptotic sensitivity induced by UV irradiation on the p53 transducted K562 cells

作　　者：陈德喜[1] 王申五[1] 曹国栋[1] 马丽萍[1] 潘秀英[1] 

机构地区：[1]北京医科大学人民医院中心实验室

出　　处：《中华血液学杂志》1998年第12期631-633,共3页Chinese Journal of Hematology

基　　金：国家自然科学基金

摘　　要：目的：观察野生型ｐ５３基因转染的Ｋ５６２细胞对紫外线诱导其细胞凋亡的影响。方法：应用基因转染技术将重组野生型ｐ５３的逆转录病毒载体转导ｐ５３蛋白缺失的Ｋ５６２细胞，用紫外线照射Ｋ５６２ｐ５３细胞不同时间后再培养不同时间，用ＤＮＡ片段化和细胞ＤＮＡ原位末端标记技术检测细胞凋亡情况。结果：紫外线照射Ｋ５６２ｎｅｏ和Ｋ５６２ｐ５３细胞８分钟后再培养７～１２小时，Ｋ５６２ｎｅｏ和Ｋ５６２ｐ５３两组细胞的凋亡差异显著。结论：野生型Ｐ５３蛋白在Ｋ５６２细胞表达后，能加速紫外线诱导的Ｋ５６２细胞凋亡。Objective:To answer whether wild type p53 can sensitize K562 cell apoptosis induced by UV irradiation. Methods: K562 cells transducted with retrovirus encoding wild type p53 were irradiated by ultraviolet for different time and then cultured for different time. Apoptosis was detected by TDT end labelling technique, DNA fragmentation and MTT assay. Results: TDT end labelling and DNA fragmentation showed that apoptosis induced by 8 min UV irradiation differed significantly between K562 neo and K562 p53 cells. Conclusion: Wild type p53 can enhance apoptosis induced by UV irradiation on K562 cells

关 键 词：白血病 细胞凋亡 P53基因 紫外线 K562细胞 

分 类 号：R733.7[医药卫生—肿瘤]