小鼠胸腺淋巴瘤动物模型的建立  

Establishment of an Animal Model with Thymic Lymphoma in Mice

作　　者：黄榕芳[1] 余英豪[1] 吴在增[1] 

机构地区：[1]南京军区福州总医院病理科,福建福州350025

出　　处：《中国实验血液学杂志》2009年第5期1220-1223,共4页Journal of Experimental Hematology

基　　金：南京军区医学科学技术研究"十一五"计划课题资助;编号06MA-147;07Z034

摘　　要：本研究通过腹腔注射N-甲基亚硝基脲(MNU)建立小鼠胸腺淋巴瘤动物模型。第0、8周2次给C57BL/6小鼠腹腔注射MNU诱导液50mg/kg体重注射后观察动物一般情况;第22周处死全部小鼠,解剖检查胸腺肿瘤的发生及其他脏器情况。结果表明:第22周时67.5%(27/40)实验小鼠产生胸腺淋巴瘤。不同性别对成瘤率影响无明显差异。结论:腹腔分次注射MNU可以诱导小鼠建立胸腺淋巴瘤动物模型,其生物学行为与人类相似。The objective of study was to establish an animal model with thymic lymphoma in mice induced by intraperitoneal injection of DNA alkylating agent N-methyl-N-nitrosourea （MNU）. Male and female mice of the C57BL/6 strain were injected by the intraperitoneal route with MNU solution in a dosage of 50 mg/kg body weight. The injection was repeated at week 8. Following injection of MNU, the general status of mice was observed. All mice were sacrificed for autopsy at the 22th experimental week. Complete gross examination was performed for detection of tumor masses. The results showed that at the 22th week, the incidence of thymic lymphoma in MNU-treated animals was 67.5% （27 / 40）. No significant sex difference in the incidence of thymic lymphoma was observed. In conclusions, an animal model with thymic lymphoma in mice can be established by twice intraperitoneal administration of MNU. The biological behavior of the induced tumors resembles to those of human thymic lymphoma derived from thymic T-cells.

关 键 词：恶性淋巴瘤 胸腺 动物模型 化学致癌剂 N-甲基亚硝基脲 

分 类 号：R733.1[医药卫生—肿瘤] R332[医药卫生—临床医学]