C反应蛋白对多发性骨髓瘤细胞系U266增殖机制的研究  

Mechanism of C-Reactive Protein on Proliferation of Multiple Myeloma U266 Cells

作　　者：杨薏蓉[1] 黄灵娟[1] 马艳萍[1] 鹿育晋[1] 杨林花[1] 周永安[2] 

机构地区：[1]山西医科大学第二医院血液科,山西太原030001 [2]山西省儿童医院血液科实验室,山西太原030001

出　　处：《中国实验血液学杂志》2009年第5期1242-1245,共4页Journal of Experimental Hematology

基　　金：山西省留学人员科研资助项目;编号20081099

摘　　要：为了观察C反应蛋白(CRP)对人骨髓瘤细胞系U266细胞增殖活性的作用机制,将液氮冻存的U266细胞复苏后,悬浮培养,第3天收集细胞备用,分别以不同浓度CRP(0、5、10、20mg/L)培养24小时,采用血液分析仪检测细胞增殖情况,采用逆转录-聚合酶链反应(RT-PCR)方法检测凋亡相关蛋白survivin和HSP90α的表达。结果发现:CRP处理的细胞组增殖率及survivin、HSP90α的表达量均高于对照组(p<0.05),20mg/LCRP组作用最显著;survivin、HSP90α之间表达具有显著相关性(r=0.737,p<0.0001)。结论:CRP是通过调节凋亡抑制蛋白Survivin及HSP90α的表达发挥促进U266细胞增殖的作用。This study was purposed to investigate the mechanism of C-reactive protein （CRP） on proliferation of U266 cells. The human multiple meyloma cell line U266 was incubated with human CRP（0,5,10,20 mg/L） for 24 hours, then the proliferation level of U266 cells was detected by using blood analyser. The mRNA expressions of survivin and HSP90α were examined by RT-PCR. The results showed that the proliferation ratio was increased, as compared with the control group（p 〈0.05 ） ; furthermore, the mRNA levels of survivin and HSP90α were up-regulated in proportion to the increased CRP concentrations. There was significant correlation between expression of survivin and HSP90et （ r = 0. 737 ,p 〈 0.0001 ） in incubated cells. It is concluded that CRP can stimulate the proliferation of MM cells directly by up-regulating the expression of survivin and HSP90α in MM cells. CRP can be regarded as a potential target for MM treatment.

关 键 词：C反应蛋白 SURVIVIN HSP90Α 骨髓瘤 U266细胞 

分 类 号：R733.3[医药卫生—肿瘤]