骨髓间充质干细胞治疗ConA诱导的小鼠急性肝损伤的有效性研究  

Therapeutic Efficacy of Bone Marrow-Derived Mesenchymal Stem Cells Infused into Mice with Liver Injury Induced by Concanavalin A

作　　者：马杰[1] 曹莹 胡建立 孙慧[1] 刘林湘[1] 赵春华 

机构地区：[1]郑州大学第一附属医院血液科,郑州450052 [2]中国医学科学院、北京协和医学院、基础医学研究所/基础医学院组织工程研究中心,北京100005

出　　处：《中国实验血液学杂志》2009年第5期1289-1293,共5页Journal of Experimental Hematology

基　　金：国家高技术研究发展计划863资助项目(编号2002AA205061);国家杰出青年基金资助项目(编号30125018)

摘　　要：为了探讨骨髓原间充质干细胞(mesenchymal stem cells,MSC)治疗急性肝损伤的可行性及有效性,本研究分离培养小鼠MSC,并建立刀豆蛋白A(concanavalin A,ConA)诱导的小鼠急性肝损伤模型。在ConA静脉注射后立即给予MSC静脉输注,24小时后进行小鼠血清转氨酶、肝脏组织病理学和原位细胞凋亡检测,并用实时定量RT-PCR的方法检测小鼠肝脏组织内炎症介质表达变化。结果显示,ConA静脉注射后立即给予MSC静脉输注,小鼠血清丙氨酸氨基转移酶(alanine aminotransferase,ALT)和天门冬氨酸氨基转移酶(aspartate aminotransferase,AST)水平降低(p<0.05),肝脏坏死和肝细胞凋亡减轻,肝脏组织内TNF-α、IFN-γ的水平明显降低(p<0.05),iNOS、IL-2和IL-10的表达水平基本不受影响(p>0.05)。结论:静脉输注MSC能减轻ConA诱导的小鼠急性肝损伤。The aim of this study was to evaluate the therapeutic potential of bone marrow mesenchymal stem cells （MSC） on acute liver injury induced by concanavalin A （ConA）. MSCs were isolated from male C57BL/6 mice and cultured, and a ConA-induced acute liver injury model was used. MSCs were systemically infused immediately after mice were challenged with ConA, control mice received only saline infusion. 24 hours after MSC transplantation, the level of serum aminotransferases, histologic change and in situ apoptosis of cells were detected, the expression of inflammatory mediators were examined by real-time RT-PCR. The results indicated that MSC transplantation significantly reduced ConA-induced acute liver injury, including the decrease of the level of serum alanine aminotransferase（ALT）, serum aspartate aminotransferase （AST） and the extenuation of liver necrosis and in situ apoptosis. Furthermore, after MSC infusion the expression of TNF-α, IFN-γ in liver decreased greatly （p 〈 0.05 ） with no statistical difference in the expression of iNOS, IL-2 and IL-10 （p 〉 0.05 ）. It is concluded that the systemic infusion of MSCs can alleviate ConA induced acute liver injury in mice.

关 键 词：骨髓间充质干细胞 刀豆蛋白A 急性肝损伤 

分 类 号：R575[医药卫生—消化系统]