三个STR位点在傣族人群中的遗传多态性  

DISTRIBUTION OF THREE STR LOCI IN DAI ETHNIC GROUP IN CHINA

作　　者：沈洪[1,2,3,4,5] 邹苹[1,2,3,4,5] 杨燕 邹浪萍[1,2,3,4,5] 申滨 

机构地区：[1]云南省红十字会医院 [2]昆明市妇幼保健医院 [3]云南省人民医院 [4]云南高级人民法院司法鉴定科学技术研究所 [5]昆明医学院

出　　处：《中华医学遗传学杂志》1998年第6期357-359,共3页Chinese Journal of Medical Genetics

基　　金：国家自然科学基金

摘　　要：目的了解云南傣族３个ＳＴＲ位点遗传多态性分布。方法应用多重ＰＣＲ技术对ＨＵＭＣＳＦ１ＰＯ、ＨＵＭＴＰＯＸ和ＨＵＭＴＨ０１３个ＳＴＲ基因座进行分析，采用高分辨率的聚丙烯酰胺凝胶电泳分离、银染法显影技术，对云南傣族上述３个基因座进行遗传多态性调查。结果ＣＳＦ１ＰＯ位点，观察到７个等位基因，１５个基因型；ＴＰＯＸ位点，观察到７个等位基因，１８个基因型；ＴＨ０１位点，观察到６个等位基因，１７个基因型。结论上述３个短串联重复序列基因座基因频率分布与Ｈａｒｄｙ－Ｗｅｉｂｅｒｇ平衡吻合良好。Objective This study was conducted to investigate the distribution of three STR loci in Dai ethnic group.Methods Multiplex amplifications of CSF1PO, TPOX and TH01 were carried out. Using denaturing polyacrylamide gel electrophoresis and silver staining the authors investigated the distribution of allele frequencies of CSF1PO, TPOX and TH01 loci in Dai ethnic group living in Yunnan province.Results 7 alleles and 15 genotypes of CSF1PO locus, 7 alleles and 18 genotypes of TPOX locus, 6 alleles and 17 genotypes of TH01 locus were observed.Conclusion The allele distribution of the loci is in good agreement with the Hardy Weinberg equilibrium.

关 键 词：遗传多态性 傣族 STR位点 短串联重复序列 

分 类 号：Q347[生物学—遗传学] Q346.5