年龄对牙周膜细胞钙化表达的影响  

Effect of age on mineralization expression of periodontal ligament cell clones

作　　者：刘宏伟[1] 马良[1] 欧龙[1] 袁志萍[1] 庞劲凡[1] 

机构地区：[1]解放军总医院口腔科

出　　处：《中华口腔医学杂志》1998年第6期329-331,共3页Chinese Journal of Stomatology

基　　金：国家自然科学基金;国家教委留学回国人员科研启动基金

摘　　要：目的探讨不同年龄人的牙周膜（ｐｅｒｉｏｄｏｎｔａｌｌｉｇａｍｅｎｔ，ＰＤＬ）细胞参与牙周组织再生的能力和影响因素。方法对青年人（４颗牙）和老年人（３颗牙）的正常牙周膜细胞进行细胞克隆化，观察碱基成纤维细胞生长因子（ｂａｓｉｃｆｉｂｒｏｂｌａｓｔｇｒｏｗｔｈｆａｃｔｏｒ，ｂＦＧＦ）对ＰＤＬ细胞克隆体外钙化能力的影响。结果青年组钙化细胞约占接种细胞数的１７７％，而老年组仅占４１％。可见老年人ＰＤＬ细胞的钙化能力明显低于青年人。当ｂＦＧＦ作用后，青年组所获钙化细胞克隆数较前增加２６３％；而老年组增加３４％。结论老年组ＰＤＬ中前体细胞的数量和对ｂＦＧＦ的生物学应答均低于青年组。对老年人牙周组织再生治疗原则和研究方向应有别于青年人，应着重于增加老年人ＰＤＬ中干细胞池的数量和激活前体细胞的生物学功能。Objective To evaluate the effect of age on the expression of mineralized tissue progenitors in the periodontium and the effect of basic fibroblast growth factor (bFGF).Methods The periodontal ligament (PDL) tissues that derived from three old and 4 young donors were cultured and cells were cloned. The mineralizing ability were detected with or without bFGF treatment in the presence of dexamethasone. The cell cloning technic was employed in present study.Results The results indicated that the frequency of mineralized tissue progenitors (old donor: 4.1%) and the numbers of cell clones that mineralized with bFGF treated in the PDL cultures derived from old donors (3.4%) is considerably less than that of cultures obtained from young donors (17.7% and 16.9%). bFGF enhanced the mineralization of cell clone but not the parent population.Conclusion It is concluded that the poor stem cell and progenitors in PDL and the low reaction of progenitors to growth factors in PDL are responsible for the limited regenerative capacity of periodontium in old patient.

关 键 词：牙周组织 年龄 钙化 牙周膜 细胞再生 

分 类 号：R78[医药卫生—口腔医学] R329.28[医药卫生—临床医学]