人γδT细胞、CD3AK细胞、CIK细胞和树突状细胞对已建立胃癌裸鼠的治疗作用  

Anti-Tumor Effects of the Human γδT Cells,CD3AK Cells,CIK Cells and DC on Nude Mice with Xenografts of Human Gastric Carcinoma

作　　者：陈复兴[1] 刘军权[1] 张南征[1] 唐涛[2] 王涛[1] 周忠海[1] 冯霞[2] 周青[2] 李玺[1] 

机构地区：[1]解放军第97医院实验科,江苏徐州221004 [2]徐州医学院附属医院检验科,江苏徐州221004

出　　处：《肿瘤基础与临床》2009年第4期277-280,共4页journal of basic and clinical oncology

基　　金：南京军区医学科学技术研究<十一五>计划课题项目基金资助(编号:06MA45)

摘　　要：目的探讨胃癌患者外周血γδT细胞、CD3AK细胞、CIK细胞和树突状细胞(DC)对已建立的胃癌裸鼠的免疫治疗作用。方法用人胃癌细胞株(SGC-7901)接种BALC/c裸鼠皮下,建立胃癌裸鼠模型。将已建立胃癌的裸鼠随机分为4组,每组5只,A组:用RPMI-1640培养液治疗作为对照组;B组:DC+CIK细胞治疗组,C组:γδT细胞治疗组;D组:DC+CD3AK细胞+γδT细胞治疗组,治疗组每次治疗同时给予IL-2 6 000单位,肿瘤细胞接种第4天开始治疗,以后每3 d进行一次治疗,共3次,从接种肿瘤细胞后第55天结束实验。结果裸鼠接种5×106胃癌细胞后第4天,在接种部位均有肿瘤形成,直径为2～3 mm,成瘤率为100%。荷瘤小鼠经第一次细胞免疫治疗3 d后B、C、D组肿瘤消退裸鼠分别为1、2、1只,第2次细胞免疫治疗后肿瘤消退的裸鼠的数目分别为2、2、2只,末消退的肿瘤体积均有减少,而对照组肿瘤体积不断增大。在第一次治疗后20 d时测A、B、C、D组平均肿瘤体积分别为87、40、45和38 mm3,各治疗组与对照组比较差异有统计学意义(P<0.01)。治疗组存活天数较对照组存活天数长,其中C组与D组存活天数比较差异有统计学意义(P<0.01),D组长于C组。结论用胃癌患者的DC+CIK细胞、γδT细胞、DC+CD3AK细胞+γδT细胞治疗胃癌裸鼠能不同程度消退裸鼠已建立的胃癌和延缓其生长。细胞免疫治疗能延长荷瘤裸鼠生存期,其中D组免疫治疗后小鼠存活天数大于C组。Objective Tumor cellular immunotherapy is a hot spot in tumor research.This study was to explore the effect of immunotherapy with γδ T cells,CD3AK cells,CIK cells and dendritic cells（DC） on patients with gastric cancer in nude mice has been established with human gastric carcinoma cell line（SGC-7901）.Methods The BALC/c nude mice was inoculated subcutaneously with human gastric carcinoma cells.The established gastric cancer in nude mice was randomly divided into four groups,each with five the group A with RPMI 1640 medium treated as a control group,the group B for DC ＋ CIK cells treated group;the group C for γδT cells treatment group;the group D for CD3AK cells ＋ DC ＋γδ T cells treated group,the treatment group immunotherapy at the same time was given IL-2 6 000 units,the fourth day of tumor cells inoculation beginning immunotherapy,every three days after treatment,a total of three times,after inoculation of tumor cells.The experiment was completed after the first 55 days.Results The nude mice inoculated with 5×106 gastric carcinoma cells,after the fourth day in the inoculation site had formed a diameter of 2-3 mm tumor,the tumor formed rate was 100%.The tumor-bearing mice for the first time in three days after immunotherapy,the B,C and Group D tumor regression 1,2,1,respectively.2nd after immunotherapy for 2,2,2,regression respectively.The disappearance of tumor volume had diminished,and tumor volume of the control group grow.In the first 20 days after treatment determined the A,B,C and D group tumor average size were 87,40,45 and 38 mm3 respectively,the treatment group with very significant difference（P〈0.01）and in comparison with the control group in the tumor size.The treatment group survived the control group several days.In the treatment group,the group D survived the group C for a few days（P〈0.01）.Conclusion The human DC ＋ CIK cells,γδT cells,DC ＋CD3AK cells＋γδ T cells therapy can regress in varying degrees established gastric carcinoma in nude mice and can delay i

关 键 词：ΓΔT细胞 CD3AK细胞 树突状细胞 CIK细胞 胃癌 免疫治疗 裸鼠 

分 类 号：R735.2[医药卫生—肿瘤]