栽培曼地亚红豆杉针叶化学成分的分离与鉴定  

Isolation and indentification of chemical constituents from the needles of Taxus media 'Hicksii'

作　　者：张娜[1] 路金才[1] 王晶[1] 韩凌 张晶晶[1] 

机构地区：[1]沈阳药科大学中药学院,辽宁沈阳110016 [2]国家中成药工程技术研究中心,辽宁本溪117004

出　　处：《沈阳药科大学学报》2009年第10期789-791,799,共4页Journal of Shenyang Pharmaceutical University

摘　　要：目的对栽培于辽宁本溪的曼地亚红豆杉(Taxus media‘Hicksii’)针叶的化学成分进行研究。方法曼地亚红豆杉针叶的乙醇提取物经大孔树脂、硅胶柱色谱、ODS柱色谱、凝胶柱色谱进行分离纯化,依据1H-NMR、13C-NMR、ESI-MS、HRESI-MS鉴定其结构。结果从曼地亚红豆杉针叶的乙醇提取物中分离纯化得到6个化合物,分别鉴定为二萜类化合物:5-cinnamoyl-9-acetyltaxicin-I(1)、5-cinnamoyl-10-acetyltaxicin-I(2)、5-去桂皮酰基紫杉宁J(3)、紫杉醇(4);黄酮类化合物:柚皮素(5);脂肪酸类:十六烷酸(6)。结论化合物1、2、5和6为首次从曼地亚红豆杉中分离得到。Objective To investigate chemical constituents from the needles of Taxus media growing in Liaoning province of China. Methods The needles of the plant were extracted with ethanol;the ethanol extract was submitted to chromatography on silica gel column, ODS and Sephadex LH-20 for the isolation. Their structures were elucidated on the basis of spectral analysis. Results Six compounds were isolated and identified as 5-cinnamoyl-9-acetyltaxicin-I （ 1 ）, 5-cinnamoyl-10-acetyltaxicin-I （ 2 ）, 5-decinnamoyltaxinine J （3）, taxol （4）, nadngenin （ 5 ）, hexadecanoic acid （ 6 ）. Conclusions Compounds 1,2,5 and 6 are isolated from Taxus media‘Hicksii＇.

关 键 词：曼地亚红豆杉 紫杉烷二萜 黄酮 脂肪酸 化学成分 结构鉴定 

分 类 号：R914[医药卫生—药物化学] R284.1[医药卫生—药学]