应用化学诱变法筛选抗草甘膦大豆突变株系  

Glyphosate Resistant Mutant Strain of Soybean Filtered by Chemomorphosis

作　　者：张俐俐[1] 谷维[2] 雷勃钧[1] 吕晓波[1] 李铁[1] 

机构地区：[1]黑龙江省农业科学院生物技术研究所,黑龙江哈尔滨150086 [2]黑龙江省农业科学院作物营养实用技术研究所,黑龙江哈尔滨150086

出　　处：《大豆科学》2009年第5期938-940,共3页Soybean Science

基　　金：黑龙江省自然基金(B类)资助项目(ZJN04-0402)

摘　　要：利用化学诱变剂甲基磺酸乙酯(EMS)和叠氮化钠,对黑龙江省大面积推广的6个农艺性状优良的大豆品种进行种子处理。不同品种按不同处理剂量分区种植,按常规法单株选择收获。不同浓度诱变剂处理大豆种子的M1代成活率均高于半致死浓度处理;对M3代35518个植株于3叶期进行喷洒1.31 ai.kg.hm2草甘膦浓度鉴定,其中40株生长正常,所获抗性植株是经5 mmol.L-1叠氮化钠诱变处理的绥农10和黑农44。2008年继续对M4代进行鉴定,抗草甘膦特性可以稳定遗传。应用化学诱变法可以在后代群体中筛选到具有抗草甘膦特性的突变株系。To screen soybean lines resistant to glyphosate through chemomorphosis, six soybean varieties widly planted in Heilongjiang Province were dealt with chemical mutagen ethyl methane sulphonate （EMS） and sodium azide by seed soaking. The survival rate of soybean seeds in M1 generation was higher than that of semi-lethal concentrations. In M3 generation,35518 plants were identified by spraying 1.31ai. kg· ha^-1 glyphosate at three-leaf stage, from which a total of 40 resistant plants showed normal growth ,and they were Suinong 10 and Heinong 44 dealt with 5 mmol · L^-1 sodium azide. The M4 generation of the 40 survival lines also showed glyphosate resistance. Results suggest chemical mutagen could be used in selecting soybean mutant lines resistant to glyphosate.

关 键 词：大豆 甲基磺酸乙酯 叠氮化钠 化学诱变 草甘膦抗性 

分 类 号：S565.1[农业科学—作物学]