宫颈鳞癌细胞中HPV和C-erbB-2基因扩增的检测  

Detection of HPV and CerbB2 Amplification in Cervical squamous Cancer

作　　者：李忠佑[1] 王建东[1] 朱月清[1] 王亚平[1] 薛开先[1] 林厚斌[1] 

机构地区：[1]江苏省肿瘤防治研究所

出　　处：《实用癌症杂志》1998年第1期21-23,共3页The Practical Journal of Cancer

基　　金：江苏省卫生厅基金

摘　　要：应用ＰＣＲ和差别ＰＣＲ方法，平行检测４０例宫颈鳞癌和６例宫颈炎细胞中的ＨＰＶ感染和Ｃ－ｅｒｂＢ－２基因扩增。结果宫颈鳞癌中ＨＰＶ混合型别（ＨＰＶＳ）感染阳性率为６５．０％（２６／４０），其中，ＨＰＶ１６占３７．５％（１５／４０），ＨＰＶ１８仅占５．０％（２／４０）；而宫颈炎中仅１例ＨＰＶｓ感染，两者具有显著性差异（χ２＝５．０１，Ｐ＜０．０５），所有宫颈癌和宫颈炎中均不见Ｃ－ｅｒｂＢ－２基因扩增。结果表明：ＨＰＶ感染与宫颈鳞癌的发生关系密切，但与Ｃ－ｅｒｂＢ－２癌基因扩增没有直接联系。Human papillomavirus and CerbB2 amplification were detected in 40 cases of cervical squamous carcinoma and 6 cases of cervicitis with PCR and DifferentialPCR methods, the positive rates of HPV mixedtypes, HPV16 and HPV18 were 65.0%,37.5%and 5.0%respectiely in cancer tissues, the positive rate of HPVs in cerviticitis was 16.7%, the difference were significant (χ2=5.01,P<0.05), but no CerbB2 amplification was found in both of them It shows that HPV has no directive effect on CerbB2 amplification in cervical squamous canncer.

关 键 词：子宫肿瘤 宫颈鳞癌 人乳头瘤病毒 基因扩增 PCR 

分 类 号：R737.33[医药卫生—肿瘤]