茶多酚清除氧自由基及抑制脑脂质过氧化反应的体外试验  

In vitro experiment on scavening oxyradical and suppressing cerebral lipid peroxidation with tea polyphenol

作　　者：何冰[1] 陈小夏[1] 

机构地区：[1]广东药学院药理学教研室

出　　处：《中国药理学通报》1998年第3期270-272,共3页Chinese Pharmacological Bulletin

摘　　要：目的研究茶多酚对羟自由基（·ＯＨ）、超氧阴离子自由基（Ｏ·２）的清除作用及其对脂质过氧化的抑制作用。方法茶多酚与自由基发生体系或大鼠脑线粒体共浴后，比色法测定·ＯＨ、Ｏ·２及丙二醛生成量。结果茶多酚对Ｆｅｎｔｏｎ反应生成的·ＯＨ及黄嘌呤－黄嘌呤氧化酶系统产生的Ｏ·２具有较强的清除作用，ＩＣ５０分别为９１９．６ｍｇ·Ｌ－１和８３６ｍｇ·Ｌ－１。茶多酚对·ＯＨ诱导的离体大鼠脑线粒体产生的脂质过氧化有明显的抑制作用。AIM To study the oxygen free radical scavenging activities and antilipid peroxidation of tea polyphenol. METHODS The scavening effects of tea polyphenol on hydroxyl radical (·OH) and superoxide anion (O·2) were inverstigated by using fenton reaction (Fe^2++H_2O_2) and xanthinexanthine oxidase system. The antilipid peroxidation of tea polyphenol as studied by using colorimetric estimation of malondialdehyde (MDA) formation. RESULTS Tea polyphenol possessed significantly scavening effects on ·OH produced by fenton reaction and O·2 produced by xanthinexanthine oxidase system (the IC50 were 9196 mg·L-1 and 836 mg·L-1 respectively). Tea polyphenol could significantly inhibit the lipid peroxidation of cerbral mitochondrial membrane induced by ·OH. CONCLUTION The antilipid peroxidation of tea polyphenol may be related to its scavenging effects on oxygen free radicals.

关 键 词：茶多酚 脑缺血 再灌注损伤 LPO 氧自由基 

分 类 号：R977.9[医药卫生—药品] R743.305[医药卫生—药学]