大鼠带蒂十二指肠壁瓣胃移植的组织学与电镜观察  

The mucosa of pediculed duodenal wall graft transplanted to the stomach in Wistar rats: Histological and electronmicroscopic observations

作　　者：李春启[1] 王建荣[1] 张秀荣[1] 李学甫[1] 步晓华[1] 胡益云[1] 

机构地区：[1]中国人民解放军空军总医院消化科

出　　处：《中华医学杂志》1998年第4期296-298,共3页National Medical Journal of China

基　　金：全军"九五"青年基金

摘　　要：目的探讨十二指肠粘膜胃上皮化生的发生机理。方法将３０只大鼠带蒂十二指肠壁瓣自体移植到胃内，于术后第３、６、９、１２个月，分批处死动物进行组织学、粘液组织化学、ＡＫＰ组织化学及电镜观察。结果术后第６、９、１２个月时，移入胃内的十二指肠壁瓣粘膜内可见呈灶状或片状分布的胃上皮化生，化生细胞的特征为含ＰＡＳ阳性中性粘液，无明显刷状缘；透射电镜和扫描电镜观察，可见十二指肠壁瓣粘膜内含有分泌颗粒的胃型上皮和稀疏排列的不规则型微绒毛；术后第９、１２个月，移入胃内的十二指肠壁瓣粘膜ＡＫＰ反应明显减弱或阴性。结论十二指肠粘膜胃上皮化生是一种微环境相关性变化，低ｐＨ值可能是诱发十二指肠粘膜胃上皮化生的主要因素之一。Objective To study the development mechanism of gastric metaplasia in the duodenum through the transplantation of a flap of duodenal wall with vascular pedicle to the stomach. Methods The pedicled duodenal wall flaps of Wistar rats were transplanted to their stomaches. After the operation the rats were killed at the 3th, 6th, 9th and 12th month respectively, and histological change of the duodenal wall grafts was observed with optical and electron microscopy. Results Gastric metaplasia was found in the mucosa of duodenal wall grafts transplanted to the stomach at the 6th, 9th and 12th month. Conclusions The formation of gastric metaplasia in the duodenal mucosa may be related to a change of the microenvironment around the tissues, and duodenal mucosa may differentiate into gastric epithelium by the decrease of pH value.

关 键 词：十二指肠粘膜 胃 上皮化生 移植 电镜 溃疡 

分 类 号：R573.102[医药卫生—消化系统]