14-3-3ζ与β连接素在人T_1期非小细胞肺癌中的表达及意义  

Expressions of 14-3-3 ζ and β-catenin proteins in human T_1 non-small cell lung cancer and the significance

作　　者：臧东钰[1] 李晓明 张林[1] 

机构地区：[1]中国医科大学附属第一临床医院胸外科 [2]组织胚胎学教研室,辽宁沈阳110001

出　　处：《解剖学报》2009年第5期826-829,共4页Acta Anatomica Sinica

摘　　要：目的观察14-3-3ζ和β连接素(β-catenin)在人T1期非小细胞肺癌(NSCLC)中的表达,探讨两者与非小细胞肺癌发展、侵袭和转移的关系。方法采用免疫组织化学、免疫荧光双标、激光扫描共焦显微技术和Western blotting,检测NSCLC及正常肺组织中14-3-3ζ和β-catenin蛋白的表达。结果与正常肺组织相比,14-3-3ζ在NSCLC中的表达显著增强,且表达与肺癌的分化程度和淋巴结转移有关,而与病理学分型无关。β-catenin在NSCLC中的表达与正常肺组织相比减弱,且其表达与肺癌的分化程度呈正相关,与淋巴结转移呈负相关,而与肺癌病理学分型无关。结论14-3-3ζ和β-catenin与T1期NSCLC发展、侵袭和转移密切相关。14-3-3ζ高表达可能促进了NSCLC的发展与转移,因此14-3-3ζ可以作为非小细胞肺癌又一个有效的治疗靶点。Objective To observe the expressions of 14-3-3ξ and β-catenin in human T1 non-small cell lung cancer （NSCLC）, and to investigate the relationship between the expressions of 14-3-3ξ and β-catenin and progression and invasion in NSCLC. Methods The expressions of 14-3-3ξ and β-catenin were detected by immunohistochemistry, double fluorescent, laser scanning confocal microscopy and Western blotting in NSCLC and normal lung. Results Up-regulation of 14-3-3ξ was observed significantly in stage TI NSCLC in contrast to that in normal lung. The increased 14-3-3ξ protein level was correlated with differentiation degree and lymph node metastasis, but it was no correlation with histological type. Decreased expression of β-catenin was observed in NSCLC in contrast to that in normal lung. The expression of β-catenin was positively related to differentiation degree and was negatively related to the lymph node metastasis, and there was no relationship between β-catenin expression and histological type. Conclusion 14-3-3ξ and β-catenin play an important role in development and progression of NSCLC. Increased expression of 14-3-3ξ possibly contributes to development and progression of NSCLC. 14-3-3ξ may also serve as an effective therapeutic target for NSCLC.

关 键 词：非小细胞肺癌 14-3-3ξ Β连接素 侵袭 转移 激光扫描共焦显微术 免疫印迹法 人 

分 类 号：R734.2[医药卫生—肿瘤]