回转器模拟失重对SGC-7901和HFE-145细胞凋亡的影响  

Effects of simulated weightlessness on apoptosis of cell line SGC-7901 and HFE-145

作　　者：朱鸣[1] 吴本俨[2] 聂捷琳[3] 李莹辉[3] 

机构地区：[1]中国人民解放军空军总医院消化内科,北京市100036 [2]中国人民解放军总医院南楼消化科,北京市100853 [3]航天医学工程研究所第13研究室,北京市100094

出　　处：《世界华人消化杂志》2009年第24期2491-2494,共4页World Chinese Journal of Digestology

基　　金：总装备部预研基金资助项目;No.51400020204JB1002~~

摘　　要：目的:探讨模拟失重对胃癌SGC-7901细胞和胃黏膜上皮HFE-145细胞凋亡的影响.方法:采用回转器模拟失重,2种细胞各分为2组,回转组和1G对照组,实验时间共72h.用TUNEL法检测细胞凋亡.结果:与对照组相比,SGC-7901细胞在72h回转组凋亡比例明显增多(P<0.01),12、24、36、48h回转组与对照组无明显差异.与对照组相比,HFE-145细胞在12h回转组凋亡比例明显增多(P<0.05),之后与对照组无明显差异.结论:回转器模拟失重使胃癌SGC-7901细胞72h时凋亡增加.胃黏膜上皮HFE-145细胞在12h时出现上述变化.AIM： To investigate the effects of simulated weightlessness on cellular apoptosis of human gastric carcinoma cell line SGC-7901 and human gastric mucosa cell line HFE-145. METHODS： A rotating clinostat was used to sim- ulate weightlessness. Each cell line was divided into two groups： rotating group and 1G control group. The experiments lasted 72 h in total. Apoptosis was measured by TUNEL method. RESULTS： Compared with control group, the apoptosis index of SGC-7901 cell line increased in rotating group in 72 h （P 〈 0.01）, but no sig- nificant change could be found in 12 h, 24 h, 36 h and 48 h. Compared with control group, the apoptosis index of HFE-145 cell line increased in 12 h （P 〈 0.05）, but no significant change was found afterward compared with control group. CONCLUSION： The apoptosis index of SGC-7901 cell line increased with simulated weightlessness by a clinostat in 72 h, while HFE-145 cells only changed in 12 h, and adapted to simulated weightlessness afterward.

关 键 词：模拟失重 胃癌细胞 胃黏膜上皮细胞 凋亡 

分 类 号：R735.2[医药卫生—肿瘤] R735.7[医药卫生—临床医学]