炎症性肠病患者肠黏膜白细胞分化抗原-14的表达及意义  

Expression of cluster of differentiation antigen-14 in intestinal mucosa of patients with inflammatory bowel disease and its implication

作　　者：吴思梦[1] 徐德魁[2] 郑长青[2] 

机构地区：[1]中国医科大学附属盛京医院输血科,辽宁省沈阳市110004 [2]中国医科大学附属盛京医院消化内科,辽宁省沈阳市110004

出　　处：《世界华人消化杂志》2009年第24期2526-2529,共4页World Chinese Journal of Digestology

摘　　要：目的:探讨炎症性肠病患者肠黏膜CD14的表达及与该病的关系.方法:收集活动性溃疡性结肠炎(ulcerativecolitis,UC)患者25例及克罗恩病(Crohn'sdisease,CD)患者15例;20例对照来自于非炎症性肠病患者手术切除的正常结肠组织(肠镜及肠黏膜病理组织学检查结果均正常).炎症性肠病的临床诊断均依据于常规影像学、内镜学及组织学标准.采用免疫组织化学方法检测肠黏膜组织中CD14表达量.结果:肠黏膜固有层单个核细胞表达CD14.UC患者CD14阳性细胞百分数高于CD患者,但二者差异无统计学意义(P>0.05);UC患者较正常对照有显著性差异(t=4.404,P<0.01),CD患者较正常对照亦见显著性差异(t=3.324,P<0.01).CD14的表达与疾病活动度(diseaseactivityindex,DAI)相关,UC组轻、中、重度比较有显著性差异(F=56.709,P<0.01);CD组轻、中、重度比较亦有显著性差异(F=12.880,P<0.01).结论:CD14参与了炎症性肠病的发病过程,其表达强度反映了该病的程度.AIM： To investigate the expression of CD14 in intestinal mucosa of patients with inflammatory bowel disease and its relationship with the disease. METHODS： Specimens from 25 cases of ulcer- ative colitis （UC） and 15 Crohn＇s disease （CD） and 20 controls were studied by immunohis- tochemical staining. The clinical diagnosis of inflammatory bowel disease was confirmed according to the routine radiologic, endoscopic and histologic criteria. All controls had normal colonoscopy, and the mucosal biopsies were his- tologically normal.RESULTS： There was expression of CD14 in lamina propria. Percent of positive cells in intes- tinal mucosa in ulcerative colitis was higher than that in Crohn＇s disease, but without significant difference （P 〉 0.05）. There was significant dif- ference between ulcerative colitis and controls （t = 4.404, P 〈 0.01）. Difference was also significant between Crohn＇s disease and controls （t = 3.324, P 〈 0.01）. CD14 was positively related to the dis- ease activity index. There was significant differ- ence among slight, moderate and severe UC （F = 56.709, P 〈 0.01） and as well as among the slight, moderate and severe CD （F = 12.880, P 〈 0.01）. CONCLUSION： CD14 may be involved in the pathogenesis of inflammatory bowel disease, and its intensity reflects the activity of the disease.

关 键 词：CD14 溃疡性结肠炎 克罗恩病 

分 类 号：R574[医药卫生—消化系统] R781.42[医药卫生—内科学]