镁对兔全脑缺血后神经保护作用的实验研究  

STUDY ON THE NEUROPROTECTION EFFECT OF MAGNESIUM SULFATE ON GLOBAL ISCHEMIA IN RABBITS

作　　者：欧册华[1] 姜鲜[1] 张毅[2] 李惠芳[2] 

机构地区：[1]泸州医学院附属医院麻醉科,泸州646000 [2]昆明医学院附属第一医院麻醉科

出　　处：《现代预防医学》2009年第21期4167-4168,4173,共3页Modern Preventive Medicine

摘　　要：[目的]观察硫酸镁预处理及硫酸镁治疗对兔全脑缺血后的神经保护作用。[方法]40只兔随机分为4组:对照组、缺血组、镁预处理组和镁治疗组,每组10只。阻断4血管(双侧颈总动脉及椎动脉),诱导全脑缺血,缺血时间为6min。测定缺血再灌注30min海马氨基酸含量。检测缺血再灌注3d海马CA1区神经元密度及凋亡神经元密度。[结果](1)镁预处理组及镁治疗组海马CA1区神经元密度显著高于缺血组(P﹤0.01),凋亡神经元密度显著低于缺血组(P﹤0.01)。(2)镁预处理组及镁治疗组海马天冬氨酸和甘氨酸含量显著低于缺血组(P﹤0.01);而γ-氨基丁酸含量显著高于缺血组(P﹤0.05)。(3)镁预处理组与镁治疗组比较各指标差异无统计学意义(P﹥0.05)。[结论]硫酸镁预处理及硫酸镁治疗对兔全脑缺血有显著的神经保护作用。[Objective] To observe the neuroprotection effect of magnesium sulfate pretreatment and magnesium sulfate therapy on global ischemia in rabbits. [Methods] Forty rabbits were divided randomly into three groups： control group （n = 10）. ischemic group（n = 10）. magnesium pretreatment group （n= 10） and magnesium therapy group （n = 10）. The global ischemia was produced by block of four vessels （both carotid and vertebral arteries） for 6 min. The density of normal neurons and apoptotic neurons in the hippocampal CAI field were measured 3 days after reperfusion. The content of amino acid in hippocampus was measured 30 min after reperfusion. [Results] （1） The group and magnesium therapy group were significantly higher than that densities of normal of ischemic group neurons in magnesium pretreatment （P 〈 0.01）, and the densities of apoptotic neurons in magnesium pretreatment group and magnesium therapy group were decreased significantly （P 〈 0.01 ）. （2） The contents of asparfic acid and glycine in magnesium pretreatment group and magnesium therapy group were significantly lower than that of isehemic group （P 〈 0.01 ）. The contents of GABA in magnesium pretearment group and magnesium therapy group were significantly higher than that of ischemic group （P 〈 0.05）. （3） There was no significance between magnesium pretreatment group and magnesium therapy group （P 〉 0.05）. [Conclusion] Magnesium sulfate pretreatment has significant neuroprotection effect on global ischemia in rabbits.

关 键 词：硫酸镁 全脑缺血 神经保护 预处理 

分 类 号：R743.3[医药卫生—神经病学与精神病学]