诱导高纯度树突状细胞的研究  

The Research of Inducing High-purity DCs

作　　者：曹天泽[1] 高维实[1] 郭慧军[1] 高艳伟[1] 施晓辉[2] 马虎林[2] 汤明忠[2] 

机构地区：[1]内蒙古自治区医院肿瘤外科,内蒙古呼和浩特010017 [2]内蒙古医学院研究生院,内蒙古呼和浩特010050

出　　处：《内蒙古医学杂志》2009年第9期1025-1028,共4页Inner Mongolia Medical Journal

基　　金：内蒙古自治区自然科学基金资助重大项目(No:200508010901)

摘　　要：目的:研究树突状细胞(Dendritic cells,DCs)的分离和诱导方法,获得高纯度树突状细胞。方法:优化外周血单个核细胞(Peripheral blood mononuclear cells,PBMCs)的分离条件,二次洗脱非贴壁细胞。应用流式细胞技术检测PBMCs中CD14+细胞纯度,检测CD80+、CD83+、CD86+及HLA-DR的阳性率确定DC纯度。结果:经流式细胞仪检测,实验组PBMCs中CD14+细胞表达率及成熟DC的CD80+、CD83+、CD86+及HLA-DR的阳性表达率显著高于对照组(P<0.05)。结论:本研究的诱导方法可得到高纯度的树突状细胞。Objective： To study the isolated and induced methods of DCs to Obtain highlypurified DCs. Method： Optimizing the centrifugation condition of PBMCs, and eluting the non - adherent cells twice. Flow cytometry （FCM） was employed to detect the purity of CDI4＋ on PBMCs and the positive rates of CD80^＋, CD83^＋, CD86^＋ and HLA- DR on mature DCs. Results： The results of FCM showed the expressions rates of CD14^＋ on PBMCs and the positive rates of CD80^＋, CD83^＋, CD86^＋ and HLA- DR on mature DCs in experiment group （induced by the improved method） is significantly higher than them in control group（P 〈 0.05）. Conclusions： The induced method can obtain highly - purified DCs.

关 键 词：树突状细胞 外周血单个核细胞 表型 

分 类 号：R730[医药卫生—肿瘤]