微水相超声波协同固定化脂肪酶催化酯交换过程优化  

Optimized Process of Biodiesel on Immobilized Lipase Catalyst Assisted with Ultrasonic Radialization in Microaqueous Media

作　　者：廖凯波[1] 曾虹燕[1] 邓欣[2] 姜和[1] 夏葵[1] 

机构地区：[1]湘潭大学化工学院,湘潭411105 [2]中国科学院西双版纳热带植物园昆明分部,昆明650223

出　　处：《天然产物研究与开发》2009年第5期871-874,865,共5页Natural Product Research and Development

基　　金：国家"863"项目(2003AA214061)

摘　　要：超声波协同固定化脂肪酶催化制备生物柴油的最佳工艺条件为:超声波功率70 W、叔丁醇为反应介质、叔丁醇用量3%(v/v)、醇油比3∶1且甲醇分三批加入、反应温度40℃、水含量为2%(v/v)。副产物甘油对固定化脂肪酶使用寿命影响最大,使用后的固定化脂肪酶用丙酮洗去表面的甘油,进行酯交换反应,酶的稳定性大为提高,可连续使用16批次。Assisted with ultrasonic radialization in mlcroaqueous media, the optimized conditions of preparation biodiesel catalyzed by the immobilized lipase were confirmed. The optimized conditions, such as ultrasonic power 70 W, n-butyl alcohol amount 3% （v/v of oil）, molar ratio of the methanol to oil 3：1,40℃, water 2% （v/v of oil） when adding methanol according to the same proportions three times,obtained a maximum ester yield 98.6%. By-product glycerol was the key point to influence the catalyst service life. If removing the glycerol on the surface of the immobilized lipase ,it could be used for 16 times.

关 键 词：固定化脂肪酶 生物柴油 超声波 微水相 酯交换 

分 类 号：R284.1[医药卫生—中药学] Q946.91[医药卫生—中医学]