鳜暴发流行病病毒性病原研究  

Identification of Outbreak and Infectious Diseases Pathogen of Siniperca chuatsi

作　　者：何建国[1] 翁少萍[1] 黄志坚[1] 曾慷[1] 

机构地区：[1]中山大学生命科学学院

出　　处：《中山大学学报（自然科学版）》1998年第5期74-77,共4页Acta Scientiarum Naturalium Universitatis Sunyatseni

摘　　要：患病鳜肾脏、脾脏、肝脏、心脏、鳃中发现二十面体病毒粒子，肾脏和脾脏是其感染主要器官．成熟病毒粒子直径１５０ｎｍ，核心球状，直径１０５ｎｍ．受感染细胞肿大，直径为正常细胞３～４倍，细胞核萎缩，为正常细胞核直径１／３．病毒粒子部分纯化后，经腹腔人工感染，第７天开始死亡，１０ｄ内死亡率为１００％，表现与池塘养殖状态相同症状，并在人工感染鳜肾和脾中电镜观察发现大量相同病毒粒子，命名为传染性脾肾坏死病毒（ｉｎｆｅｃｔｉｏｕｓｓｐｌｅｅｎａｎｄｋｉｄｎｅｙｎｅｃｒｏｓｉｓｖｉｒｕｓ，ＩＳＫＮＶ）．A virus was found in spleen, kidney, heart, gut, gill and liver of moribund cultured Siniperca chuatsi from Guangdong province by lightmicroscope and electron transmission microscope. The virus has icosahedral symmetry, and is 150 nm in diameter. The main organs of the virus infection are spleen and kidney of Siniperca chuatsi , and it caused spleen and kindey necrosis. Experimental infection identify that virus is pathogen of outbreak and infectious disease of cultured Siniperca chuatsi , we named it infectious spleen and kidney necrosis virus(ISKNV).

关 键 词：鳜 病毒 传染性 脾肾坏死病毒 ISKNV 

分 类 号：S941.41[农业科学—水产养殖]