骨癌痛大鼠脊髓背角蛋白激酶Cγ表达的变化  

Changes in expression of PKCγ in spinal dorsal horn in a rat model of bone cancer pain

作　　者：肖兴鹏[1] 曹菲[1] 杨少兵[1] 高峰[1] 田学愎[1] 陈莎莎 刘希江[1] 田玉科[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院麻醉科,武汉市430030

出　　处：《中华麻醉学杂志》2009年第9期782-784,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金资助项目（30672027）;高等学校博士学科点专项科研基金资助项目（20060487046）

摘　　要：目的探讨骨癌痛大鼠脊髓背角蛋白激酶Cγ（PKCγ）表达的变化。方法成年雌性Wistar大鼠48只，随机分为3组（n=16）：对照组、假手术组和骨癌痛组。对照组不给予任何处理，骨癌痛组右侧胫骨上部骨髓腔内注射Walker256乳腺癌细胞10μl制作骨癌痛模型；假手术组同部位注射热灭活的Walker256乳腺癌细胞10μl。各组于术前1d、术后3、6、9、12、15、18和21d时测定术侧后爪机械痛阈，并于术前1d、术后6、15和21d时各处死4只大鼠，取L4-6脊髓组织，采用免疫组化法测定脊髓背角PKCγ的表达水平。结果与术前1d时比较，骨癌痛组术后PKCγ表达上调，术后15d时达最大值（P〈0．05）；与对照组和假手术组比较，骨癌痛组术后机械痛阈降低，PKCγ表达上调（P〈0．05）；PKCγ表达水平与机械痛阂呈负相关（r=-0．984，P〈0．05）。结论骨癌痛大鼠脊髓背角PKCγ表达上调，该变化可能参与了骨癌痛的维持。Objective To investigate the changes in expression of protein kinase Cγ （PKCT） in the spinal dorsal horn in a rat model of bone cancer pain （BCP）. Methods Forty-eight female adult Wistar rats weighing 180-200 g were randomly divided into 3 groups （n = 16 each）： group I control （group C）; group II sham operation （group S） and group III. BCP. In group BCP Walker 256 mammary gland cancer cell suspension 10 μl was injected into left tibia, while in group S heat-inactivated Walker 256 mammary gland cancer cell suspension 10 μl was injected into the same region. Mechanical pain threshold of hind paw was measured every 3 d from 1 d before operation to 21 d after operation, and then the rats were killed at 1 d before and 6, 15 and 21 d after operation （4 rats at each time point） and the L4.6 segment was removed for determination of PKCγ expression （ by immuno-histochemistry）. Results PKCγ expression was up-regulated after operation and reached the highest value at 15 d after operation as compared with that at 1 d before operation in group BCP （ P 〈 0.05） . Mechanical pain threshold was significantly decreased and PKCγ expression was up-regulated in group BCP as compared with group C and S （ P 〈 0.05） . PKCγ expression was negatively correlated with mechanical pain threshold （ r = - 0. 984, P 〈 0.05）. Conclusion The expression of PKCγ is up-regulated in spinal dorsal horn in the rat with BCP, the up-regulation of PKGγ expression may be involved in the maitenance of BCP.

关 键 词：骨肿瘤 疼痛 蛋白激酶C 脊髓 

分 类 号：R738.1[医药卫生—肿瘤]