食管癌与O^6-甲基鸟嘌呤-DNA甲基转移酶基因多态性的关系  

Relationship between esophageal cancer and genetic polymorphisms of O^6-methylguanine-DNA methyltransferase gene

作　　者：余亮[1] 伊力亚尔·夏合丁 吕国栋[1] 马文静[1] 霍奇[1] 卢晓梅 

机构地区：[1]新疆医科大学第一附属医院医学研究中心,乌鲁木齐830054 [2]新疆维吾尔自治区食管癌研究所

出　　处：《中华实验外科杂志》2009年第11期1429-1431,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30760223、30860097）;新疆维吾尔自治区高技术研究发展计划资助项目（XJKJT20051113）

摘　　要：目的探讨O^6-甲基鸟嘌呤-DNA甲基转移酶（MGMT）基因多态性与新疆哈萨克族食管癌的关系。方法应用聚合酶链反应-限制性片段长度多态性（PCR—RFLP）技术检测160例新疆哈族样本（51例食管鳞状细胞癌及109例对照）的MGMT基因5个单核苷酸多态性（SNPs）的基因型。结果Promoter 485C〉A等位基因在两组间的分布经Logistic模型计算，差异有统计学意义（P〈0．05）；5个SNPs联合分析，0突变等位基因和1～10突变等位基因在两组间的差异有统计学意义（P〈0．05）。结论MGMT基因Promoter 485C〉A与新疆哈族食管癌相关；5个SNPs的联合作用与新疆哈族食管癌相关，突变等位基因增加了患食管癌的病风险。Objective To explore relationship between esophageal cancer and genetic polymorphisms of O^6-methylguanine-DNA methyltransferase （MGMT） gene in Kazakh in Xinjiang. Methods The 5 SNPs of MGMT gene in 51 esophageal squamous cell cancer （ESCC） patients and 109 controls of Kazakh in Xinjiang,were genotyped by PCR-RFLP based on case-control study strategy. Results logistic regression analysis showed that the proportion of allele for promoter 485C 〉 A in ESCC group was different from that in control group （ P 〈 0.05 ）. Combined analysis of the 5 SNPs, indicated that the proportion of the 0 allele variant and 1-10 allele variants in ESCC group was different from that in control group （ P 〈 0.05）. Conclusion Promoter 485C 〉 A of MGMT gene was associated with ESCC, and the rest 4 SNPs may be not with ESCC. The 5 SNPs of MGMT gene may jointly poly a role in the susceptibility of ESCC in Kazakh in Xinjiang, and allele variants increased risk of the occurrence of ESCC.

关 键 词：食管癌 O^6-甲基鸟嘌呤-DNA甲基转移酶 单核苷酸多态性 哈萨克族 

分 类 号：R735.1[医药卫生—肿瘤]