RNA干扰E2F-1表达对胃癌MGC803细胞生物学行为的影响及其机制  

Effects of RNA interfering E2F-1 gene expression on biological behavior of gastric cancer cell line MGC803

作　　者：苑汐子[1] 尹永硕[1] 李雷[1] 谢玉波[2] 王晓通[1] 肖强[1] 

机构地区：[1]广西医科大学第一附属医院胃肠腺体外科,南宁530021 [2]广西医科大学第一附属医院麻醉科,南宁530021

出　　处：《中华实验外科杂志》2009年第11期1467-1469,共3页Chinese Journal of Experimental Surgery

基　　金：国家自然科学基金资助项目（30860273）;广西科学基金资助项目（桂科自0640085）;广西医疗卫生重点科研资助项目（重200912）

摘　　要：目的利用基因表达谱芯片技术探讨E2F-1小分子干扰RNA（E2F-1-siRNA）影响胃癌细胞MGC803生物学行为的分子机制。方法分别抽取E2F-1-siRNA转染组和对照组（negative control—siRNA）的细胞总RNA，分离纯化mRNA并逆转录合成荧光分子（Cy3／Cy5）标记cDNA探针，与含有21522条人类22k基因表达谱芯片进行杂交。采用LuxScan 10K／A双通道激光扫描仪扫描芯片上两种信号，应用LuxScan3．0图像分析软件对芯片图像进行处理和分析。结果在21522条基因中，两组胃癌细胞间差异性表达基因为18条，其中上调基因8条，下调基因10条，下调的基因中有1条功能信息不明。结论体外干扰E2F-1基因表达后，胃癌MGC803细胞生物学行为的变化是多基因相互作用、多种信号通路相互调节的结果，其中的关键基因和信号传导通路值得进一步研究。Objective To investigate the effects of E2F-1-siRNA on biological behaviors of gastric adenocarcinoma cell line MGC803 by cDNA microarray. Methods The total RNA was extracted from gastric adenocarcinoma cell line MGC803 transfected with E2F-1-siRNA and negative control-siRNA and then purified. The cDNA was obtained by reverse transcription polymerase chain reaction （RT-PCR） ,and then labeled with Cy5 and Cy3 fluorescence as probes, which were hybridized with gene chip containing 215222 human 22k gene expression profile. Subsequently,the two signal images were scanned by LuxScan 10K/A dual pathways laser scanner and analyzed by LuxScan3.0 image analysis software. Results Of the 215222 target genes, 18 genes were screened out for differences in gene expression level between these two groups. Eight of the 18 genes were up-regulated and 10 were down-regulated, of which one had unknown function respectively. Conclusion After RNA interfering E2F-1 in vitro,the change of the gastric adeno- carcinoma cellular biological behavior is resulted from the interaction between multiple genes and regulation in signal pathways and signal transduction pathway deserve further investigation.

关 键 词：胃癌 RNA干扰 基因芯片 基因表达 

分 类 号：R735.2[医药卫生—肿瘤]