大鼠肺缺血再灌注损伤长期存活模型的建立  

Establishment of a rat lung ischemia-reperfusion injury long-term survival model

作　　者：冯冬杰[1] 蒋峰[1] 任斌辉[1] 张治[1] 王中秋[1] 尹荣[1] 许林[1] 

机构地区：[1]南京医科大学附属江苏省肿瘤医院胸外科 ,210009

出　　处：《中华实验外科杂志》2009年第11期1557-1559,共3页Chinese Journal of Experimental Surgery

基　　金：江苏省社会发展重大项目基金资助项目（BS2006005）;“333高层次人才培养工程”科研资助项目

摘　　要：目的建立一种简单、稳定的大鼠肺缺血再灌注损伤（LIRI）长期存活模型。方法将84只健康SD大鼠随机分为2组：Ⅰ组为假手术组，Ⅱ组为肺缺血再灌注组（每组n=42只）。两组分别于开胸后、缺血1h后再灌注0、2、4h、1、3、7d取肺组织行髓过氧化物酶（MPO）活性、肺湿干比（W／D ratio）检测和肺泡Ⅱ型细胞（ATⅡ）的电镜超微结构评价；取支气管肺泡灌洗液检测肺通透性指数（LPI）。结果手术成功率达100％。Ⅱ组与Ⅰ组比较，缺血再灌注后2、4h、1d的MPO、LPI、W／D比差异均有统计学意义（P〈0．01），开胸后、再灌注后0h、3、7d两组比较差异无统计学意义（P〉0．05）。ATⅡ的超微结构显示，再灌注4h后损伤最严重，再灌注1d即出现明显的修复过程，再灌注7d基本恢复正常结构水平。结论本模型简单、可靠，LIRI后相关肺损伤指标和ATⅡ超微结构损伤变化特点吻合。Objective To establish a simple and stable rat lung ischemia-reperfusion （LIRI） long-term survival model. Methods Eighty-four healthy SD rats were randomly divided into two groups ： Group Ⅰ,the sham-operated group （n = 42, no hilar blocking） ;Group Ⅱ, lung ischemia reperfusion group （n = 42,left hilar blocking）. The lung tissues and blood samples were collected at baseline before hilar occlusion,and 0 h,2 h,4 h,1 day,3 days and 7 days after reperfusion. The indices were determined as follows ： the myeloperoxidase （MPO） activity, wet/dry ratio （ W/D ratio） ,lung permeability index （LPI） and the uhrastructure of AT Ⅱ. Results All the SD rats survived during the observation period. Compared with group Ⅰ, the MPO activity, W/D ratio, and the LPI were significantly increased in group II at the time points of 2 h,4 h, 1 day after reperfusion （ all P 〈 0.01 ）. The uhrastructure of AT Ⅱ displayed that the most serious injury occurred at the tine point of 4 h after reperfusion. The repair process emerged at 1st day after reperfusion. At 7th day after reperfusion, the lung tissue structure almost recovered to normal. Conclusion It is a simple and reliable animal model. The related indices of lung injury were in accordance with the changes of uhrastruture of AT Ⅱ.

关 键 词：肺缺血 再灌注损伤 肺泡 模型 动物 

分 类 号：R-332[医药卫生]