温度两阶段控制策略发酵生产重组角质酶  

Effect of Two-staged Temperature Strategy on Production of Cutinase with Recombinant Bacillus subtilis

作　　者：张芙华[1,2] 华兆哲[1] 陈坚[1,2] 吴敬[1,2] 

机构地区：[1]江南大学生物工程学院和工业生物技术教育部重点实验室,无锡214122 [2]江南大学食品科学与技术国家重点实验室,无锡214122

出　　处：《应用与环境生物学报》2009年第5期730-733,共4页Chinese Journal of Applied and Environmental Biology

基　　金：教育部新世纪优秀人才支持计划(No.NCET-06-0486);国家高技术研究发展计划资助项目("863"计划;No.2009AA02Z204);江苏省六大人才高峰支持计划(No.08-B-吴敬);食品科学与技术国家重点实验室科研基金(No.SKLF-MB-200802);江南大学创新团队项目(No.2008CXTD01)资助~~

摘　　要：为进一步提高重组Bacillus subtilis WSHB06-07发酵生产角质酶的产量和生产强度,在pH两阶段控制策略的基础上,考察了温度(27～40℃)对菌体生长和产酶的影响.研究发现,37℃适于菌体生长而30℃适于菌体产酶.通过分析发酵过程中菌体比生长速率及产物比合成速率的变化,确定了温度两阶段控制策略,即0～4h时控制温度37℃,4h后将温度调至30℃.通过采用这一优化策略,角质酶酶活和生产强度分别可达312.5U/mL和13.02kUL-1h-1,相比恒定温度37℃控制模式下分别提高了83.4%和10.9%.On the basis of the previously published two-staged pH control strategy,the effect of temperature（from 27 ℃ to 40 ℃） on cell growth and product formation was investigated to further enhance the yield and productivity of cutinase by recombinant Bacillus subtilis WSHB06-07.The optimum temperature for cell growth was found at 37 ℃,while the optimum temperature for enzyme production was at 30 ℃.Through the analysis on the profile of the specific cell growth rate and specific cutinase formation rate,a two-stage temperature strategy, in which temperature was controlled at 37 ℃ for the first 4 h and then switched to 30℃ till the end of the fermentation, was developed. By using this temperature-shift strategy, the maximal cutinase activity and productivity reached 312.5 U/mL and 13.02 kU L^-1 h^-1, which increased by 10.9% and 83.4% respectively, compared to the constant temperature operation at 37℃. Fig 6, Tale 2, Ref 13

关 键 词：角质酶 重组枯草芽孢杆菌 温度两阶段控制 比生长速率 比合成速率 

分 类 号：TQ925[轻工技术与工程—发酵工程]