Ets-1 mRNA、Ets-1及VEGF在膀胱移行细胞癌中的表达及意义  

Expression of Ets-1 mRNA, Ets-1 and VEGF in Bladder Transitional Cell Carcinoma

作　　者：杨庞[1] 杨罗艳[2] 

机构地区：[1]江西省南昌市第一医院泌尿外科,江西南昌330008 [2]中南大学湘雅二医院,湖南长沙410011

出　　处：《医学临床研究》2009年第10期1806-1808,共3页Journal of Clinical Research

摘　　要：【目的】研究Eta-1 mRNA、Eta-1及血管内皮生长因子（vascular endothelial growth factor，VEGF）在膀胱移行细胞癌（BTCC）中的表达及意义。【方法】用原位杂交和免疫组化方法分别检测40例BTCC组织和12例正常膀胱黏膜组织中Ets—1 mRNA、Ets-1及VEGF的表达；分析Eta-1 mRNA、Ets-1表达与BTCC临床病理特征间的关系；研究BTCC中VEGF表达与Ets-1 mRNA、Ets-1表达的相关性。【结果】①Ets—1 mRNA、Ets-1在BTCC中高度表达，其表达阳性率分别为87．5％（35／40）和82．5％（33／40）（P〈0．01），且随BTCC临床分期及病理分级的升高而增加，肿瘤复发组高于无复发组，肿瘤转移组高于无转移组（P〈0．05）。②VEGF在BTCC中阳性表达率为72．5％（29／40），而对照组无一例阳性表达（P〈0．01）。在BTCC中Eta-1 mRNA、Ets-1表达与VEGF表达均呈正相关（相关系数Rs分别为0．707和0．797，P值均〈0．01）。【结论】①Ets-1 mRNA、Ets-1在BTCC中高度表达，并随BTCC分期分级升高而增加；Ets-1 mRNA、Ets-1蛋白与BTCC转移及复发密切相关。②BTCC中Eta-1 mRNA及Ets-1与VEGF表达均呈正相关，VEGF可诱导Ets-1表达。[Objective] To investigate the expression of Ets-1 mRNA, Ets-1 and vascular endothelial growth factor （VEGF） in bladder transitional cell carcinoma （BTCC）. [Methods] Samples of 40 human BTCC and 12 normal bladder mucosa tissues were examined with in situ hybridization method for Ets-1 mRNA and standard immunohistochemieal analysis for gts-1 and VEGF. The correlation between the clinical data and expression of Ets-1 mRNA and Ets 1 was analyzed. The correlations between Ets-1 mRNA, Ets-1 and VEGF were investigated. [Results]The positive rates of Ets-1 mRNA and Ets- 1 in BTCCs were 87.5% and 82.5%, respectively, which were significantly higher in BTCC specimens than those in normal bladder mucosa tissues （ P 〈0.05）. The expressions of Ets-1 mRNA and Ets-1 in BTCCs which were poorly differentiated, invasive, recurring or had lymph node metastasis were higher than those in BTCCs which were well differentiated, su perficial, recurrence-free or had no lymph node metastasis（ P 〈0. 05）. The level of VEGF expression in BTCCs was higher than that in control groups （ P 〈0.01）. Significant correlations were found between Ets 1 mRNA, Ets-1, and VEGF （Rs, Correlation Coefficient, was 0. 707 and 0. 797, respectively, P 〈0. 01）. [Conclusion]Ets-1 is expressed in BTCC and acts as a proto-oncogene which correlates closely with tumor grades, clinical stages, recurring and metastasis. A positive correlation is found between Ets-1 and VEGF in BTCC. The expression of Ets-1 is induced by VEGF.

关 键 词：膀胱肿瘤/病理学 癌 移行性细胞/病理学 RNA 信使 内皮生长因子/分析 

分 类 号：R737.14[医药卫生—肿瘤]