Snail对宫颈癌细胞侵袭转移的影响  

Effect of Snail on the invasion and metastasis of cervical cancer cells

作　　者：纪腾[1] 夏曦[1] 胡问成[1] 方勇[1] 王世宣[1] 周剑峰[1] 卢运萍[1] 马丁[1] 

机构地区：[1]华中科技大学同济医学院附属同济医院肿瘤生物医学中心,武汉430030

出　　处：《华中医学杂志》2009年第5期225-228,共4页Central China Medical Journal

基　　金：国家杰出青年基金资助项目(No.30025017);国家科技部"973"重点研究发展计划(No.2002CB513100)

摘　　要：目的研究Snail与宫颈癌侵袭转移间的关系,并探讨其分子机制。方法构建正义全长Snail真核表达载体并转染宫颈癌细胞系HeLa、SiHa。采用Western blot方法分析相关基因表达,细胞伤痕愈合实验,Trans well模型穿膜细胞计数检测细胞侵袭转移能力。结果①在转染pEGFPC1/Snail的宫颈癌细胞株HeLa、SiHa中E-cadherin表达明显下调,Snail和Vimentin则表达上调(P<0.05)。②宫颈癌细胞系HeLa、SiHa转染正义全长Snail真核表达载体,12h细胞伤痕愈合能力显著提高(P<0.05);转染pEGFPC1/Snail后,HeLa、SiHa细胞12h穿膜细胞数明显增多(P<0.05)。Snail过表达可显著促进宫颈癌细胞株HeLa、SiHa的侵袭转移潜能。结论转录因子Snail促进宫颈癌上皮细胞间质化转变(EMT),并提高其侵袭转移能力。Objective To study the relationship of Snail with invasion and metastasis of cervical cancer, and to explore its molecule mechanism. Methods Snail over-expression plasmid was transfeeted into cervical cancer cell lines of HeLa and SiHa. Western blot was applied to analyze the corresponding molecules including Snail, E-cadherin and Vimentin. The invasion and metastatic ability was examined by in vitro cell wound healing experiment and Boyden chamber invasion assay. Results ① After transfection of pEGFPC1/Snail into cervical cancer cell lines of HeLa and SiHa, the expression of E-cadherin was significantly lower than its counterpart, however, the expression of Snail and Vimentin was significantly higher than its control group. ②The capability of wound closure in SiHa and HeLa cells transfected with pEGFPC1/Snail at 12 h was significantly enhanced （P〈0.05）, and the invading/migrating cells were obviously increased at 12 h after transfection of pEGFPC1/Snail into SiHa and HeLa cells （P〈0. 05）. Up-regulation of Snail could enhance the capability of metastasis and invasion. Conclusion Transcription factor of Snail could enhance the potential of invasion and metastasis of cervical cancer cell lines through induction of Epithelial-mesenchymal transition （EMT）.

关 键 词：SNAIL 宫颈癌 上皮细胞间质化转变 上皮细胞钙黏蛋白 

分 类 号：R737.33[医药卫生—肿瘤] R734.2[医药卫生—临床医学]