猴痘病毒荧光定量PCR检测方法的建立  

Establishment of real-time PCR assays for detection of monkeypox virus

作　　者：朱娜[1] 陈国强[2] 张敬友[2] 范红结[1] 

机构地区：[1]南京农业大学动物医学院,江苏南京210095 [2]江苏出入境检验检疫局,江苏南京210001

出　　处：《南京农业大学学报》2009年第4期165-168,共4页Journal of Nanjing Agricultural University

基　　金：国家质检总局科学基金项目(2006IK011)

摘　　要：根据GenBank发表的猴痘病毒(AF380138)F3L基因全序列,设计并合成了引物和TaqMan、MGB探针,建立了F3L基因的荧光定量PCR检测方法。应用该方法可从人工合成的猴痘病毒F3L基因片段(48 048～48 509 bp)扩增典型的"S"型曲线,25μL反应体系检测灵敏度可达6.8拷贝,但不能从鸡痘、山羊痘等病毒中扩增出相应的扩增曲线。结论:本方法可快速、敏感、特异地检测猴痘病毒。In this paper the primers and TaqMan, MGB probes were designed by using Primer Express 3.0 software according to the entire F3L gene sequence of monkeypox virus in GenBank to develop a real-time PCR assay for rapid identification of monkeypox virus. A typical ＂S＂ curve can be got when the artifical F3L gene sequence was used as a target, and the detection limits were 6.8 copies of target DNA per PCR vial. However, when the chickenpox, goalpex or any other virus were used as targets, a typical ＂S＂ curve can not be got. So this assay may represent a battery of tests in detection of monkeypox virus.

关 键 词：猴痘病毒 荧光定量PCR 检测 

分 类 号：S852.659.1[农业科学—基础兽医学]