角倍蚜mtDNA Cyt b基因遗传多样性分析  

Genetic Diversity among Schlechtendalia chinensis Individuals Revealed by Cyt b Sequences

作　　者：李继变[1] 任竹梅[1,2] 

机构地区：[1]山西大学生命科学与技术学院,太原030006 [2]复旦大学生命科学学院,上海200433

出　　处：《复旦学报（自然科学版）》2009年第5期680-686,共7页Journal of Fudan University：Natural Science

基　　金：国家自然科学基金资助项目(30670361);中国博士后科学基金资助项目(2005037485);山西省自然科学基金资助项目(2007011078)

摘　　要：测定了中国1 0个不同地域角倍蚜（Schlechtendalia chinensis）1 3 2个个体的mtDNA Cyt b基因部分序列、比较其同源性、计算核苷酸组成及其变异指数、分析其种群遗传变异及遗传结构、并构建不同单倍型之间的网络和聚类图.在测得的4 3 2 bp Cyt b基因序列中有4 1个核苷酸位点存在变异（占所测核苷酸序列的9.5%）,T、C、A、G 4种核苷酸的组成分别为4 5.5%、1 4.4%、3 1.5%和8.6%,具有较高的A＋T含量（7 7.0%）;1 3 2个角倍蚜个体共产生3 1个单倍型,其中单倍型M1M4为主体单倍型,各单倍型在种群间分布不平衡,单倍型多样度平均为0.8 2 5±0.0 2 2.角倍蚜种群内各序列平均核苷酸差异数为2.2 2 7,核苷酸多样度为0.0 0 5 2±0.0 0 0 6,遗传多态性不高.AMOVA分析显示角倍蚜种群内的遗传变异（7 6.5 0%）高于种群间的遗传变异（2 3.5 0%）,FST统计结果表明我国角倍蚜种群间存在一定程度的遗传分化.基于单倍型的系统树和网络关系图显示,除个别单倍型外,所有各种群的单倍型在系统聚类.树中基本表现出一种平行式的分布格局,即单倍型并没有按地理分布形成明显的簇群,M1M4可能为原始单倍型.The genetic diversity and genetic structure of Schlechtendalia chinensis populations from China were investigated using partial mitochondrial cytochrome b sequences（432bp）.A total of 31 haplotypes were detected based on sampling 132 individuals across ten populations,in which haplotype M1-M4 were common haplotypes.The average diversity of haplotypes was 0.825±0.022.The average nucleotide diversity were 0.0052±0.0006,which indicate low gentetic diversity.The molecular variance analysis（AMOVA） revealed strong genetic structure among populations（FST= 0.2351,P〈0.05）.The NJ tree and reduced median network suggested that different individuals from the 10 areas in China were distributed in different clusters,which the branches of clusters are parallel,and there are no evident clusters between different areas,M1-M4 may be original haplotypes.

关 键 词：角倍蚜 MTDNA CYT B基因 DNA序列 多态性 

分 类 号：Q111[生物学—普通生物学]