高致龋变形链球菌探针检测与龋病现状的关系  

Identification of gtfB DNA Probe for Streptococcus mutans

作　　者：吴文[1] 耿海霞[1] 

机构地区：[1]济宁医学院临床学院,山东济宁272013

出　　处：《广东牙病防治》2009年第9期419-421,共3页Journal of Dental Prevention and Treatment

摘　　要：目的探讨唾液中gtfB基因片段检出率与龋病的关系,为寡核苷酸探针作为一种筛检方法用于临床奠定基础。方法以龋失补牙数(decayed missing of filled teeth,DMFT)记录439名不同龋敏感者口腔龋患现状,将其唾液样本中的菌体DNA抽提后行PCR扩增,其产物与gtfB探针行斑点杂交,记录阳性结果。使用SPSS 11.0统计软件计算DMFT和PCR扩增后探针杂交阳性结果之间的spearman等级相关系数。结果DMFT和PCR扩增后探针杂交阳性结果之间有密切相关性,r=0.914,P<0.01。结论变形链球菌毒力因子gtfB寡脱氧核苷酸探针敏感性高而且特异性好,且PCR扩增后探针杂交阳性结果与受试个体龋病现状密切相关,可以进一步考察该探针检测结果与受试人群未来患龋风险的相关性。Objective To design the digoxlgenin-labelled 50 bp oligonucleotide probe, complementary to the virulence factor of Streptococcus mutaus GS-5 gtfB, synthesize by AuGCT Biotechnology Company, and detect its sensitivity and specificity. Methods The PCR amplified DNA of Streptococcus mutans ATCC25175 and other conventional bacteria in human mouth was dot-blotted with the oligonucleotide probe. Results The gtfB DNA probe of Streptococcus mutans could detect homologous target DNA fixed to nylon membrane low to lng sensitivity. It could detect effectively Streptococcus mutans without cross-reaction with another six oral bacteria. Conclusion The virulence factor gtfB oligonucleotide probe of Streptococcus mutans is not only high sensitive but also specific, so it can be used in large scale epidemic investigation to screen caries susceptible. At the same time this method makes base of live examination for Streptococcus mutans.

关 键 词：变形链球菌 寡核苷酸探针 独立因子GtfB 龋齿 

分 类 号：R781.1[医药卫生—口腔医学]