鲤鱼野生群体线粒体部分基因片段序列比较  

SEQUENCE COMPARISON OF WILD COMMON CARP POPULATIONS BASED ON PARTIAL MITOCHONDRIAL DNA SEQUENCES

作　　者：赵莹莹[1,2] 匡友谊[1] 孙效文[1] 

机构地区：[1]中国水产科学院黑龙江水产研究所,哈尔滨150076 [2]沈阳农业大学畜牧兽医学院,沈阳110161

出　　处：《动物分类学报》2009年第4期812-815,共4页Acta Zootaxonomica Sinica

基　　金：国家重大基础研究(973)项目(2004CB117405);国家科技基础条件平台项目(2006DKA30470-005)资助

摘　　要：通过对线粒体16S rRNA基因、D-loop区和Cyt b基因部分片段测定,探讨我国鲤鱼C.carpio野生群体和云南大头鲤C.pellegrini的遗传变异和亲缘关系。数据和并后用于分析的序列共1 492bp,变异位点221个,含简约信息位点217个,其中新疆群体用于分析的序列长度为1 478bp。以云南大头鲤为外群,推测鲤鱼群体间的系统发育关系和分化时间,MP法和NJ法得到相似的系统树,新疆群体与其他群体的差异较大,遗传距离较远,单独聚为一支,其他群体间相似性较高聚在一起,新疆群体与其他群体间的分化时间约为2.42×10^6-2.45×10^6年前。Nucleotide sequences of 16S rRNA, D-loop,and Cyt b gene of mitochondrial DNA were amplified using PCR techniques and analyzed from wild common carp （C. carpio） populations and Barbless carp （C. pellegrini）to detect their genetic variability and phylogenetic relationship. The results showed that, 221 nucleotide variable positions and 217 parsimony-informative sites were detected in analyzed 1492 bp sequence among the populations. With Barbless carp as the out group, phylogenetic relationships among populations were reconstructed and the divergence time was estimated. The phylogenefic trees constructed by MP and NJ method were of very similar topology. Xinjiang population was different from the other populations with farther genetic distance. The populations without Xinjiang population were similar and clustered together. The divergence time between Xinjiang population was estimated about 2.42-2.45 million years ago.

关 键 词：鲤鱼 线粒体DNA 16S RRNA D-LOOP Cytb. 

分 类 号：S917.4[农业科学—水产科学]