荷瘤大鼠射频消融治疗前后血清VEGF表达水平的变化及意义  

Changes of VEGF Expression Level of Rats with Walker-256 Tumor Treated by Radiofrequency Ablation

作　　者：戴维德[1] 范智慧[2] 陈敏华[2] 河福金[2] 李洪民[2] 

机构地区：[1]卫生部北京医院超声科,北京市100730 [2]北京肿瘤医院超声科

出　　处：《中国超声医学杂志》2009年第9期835-837,共3页Chinese Journal of Ultrasound in Medicine

基　　金：基金项目：北京市科委重大项目培育专项（No.Z000519004031）

摘　　要：目的 探讨射频消融（RFA）治疗大鼠肝肿瘤对其血清血管内皮生长因子（VEGF）的影响及其意义。方法采用瘤块包埋法制作SD大鼠walker-256肝肿瘤模型30只，随机分为RFA1周组、RFA2周组和对照组，每组10只，前两组分别在RFA后1周、RFA后2周处死，对照组不做RFA即处死并取外周血，应用ELISA法检测血清VEGF。结果对照组大鼠血清VEGF水平（333．45±8．0）pg／ml。RFA后1周为（264．62±11．86）pg／ml，RFA后2周为（265．77±25．53）pg／ml，RFA后1周组及RFA后2周组与对照组比较差异均有统计学意义。结论 RFA通过对肿瘤组织的原位灭活在一定程度上解除肿瘤组织所释放的VEGF对荷瘤宿主的免疫抑制状态，促进荷瘤宿主体内树突状细胞（dendritic cells，DC）的分化成熟，提高机体抗原提呈能力。Objective To discuss the changes of serum VEGF in peripheral blood of rats with liver tumor treated by RFA. Methods Thirty Experimental liver tumor models of SD rats were prepared by implantation of tumor particles. These rats were randomly divided equally into three groups （1w after RFA, 2w after RFA and control group）. Peripheral blood of control group, group 1w after RFA and group 2w after RFA was taken out respectively. The ex pression level of serum VEGF were deetected by ELISA. Results The serum expression level of VEGF of control group was （333.45± 8.0） pg/ml, and that of group 1 week and 2 week after RFA were respectively （264.62± 11.86） pg/ml and （265.77±25.53） pg/ml. There was statistical significance of the difference between control group and group 1 week or 2 week after RFA. Conclusions （1） RFA can destroy the tumor tissues in situ and relief im mune suppression by VEGF released by tumor tissue. （2） RFA can improve differentiation and maturation of DC in local area of tumor and promote the ability of body antigen-presentation.

关 键 词：射频消融 大鼠 肝肿瘤 VEGF 树突状细胞 

分 类 号：R734.2[医药卫生—肿瘤] R735.7[医药卫生—临床医学]