环氧化酶-2在肝细胞肝癌中的表达及与多药耐药的相关性研究  

Study on expression of cyclooxygenase-2 protein in hunan hepatocellular carcinoma and correlation with multidrug resistance

作　　者：刘勇[1] 张伟[1] 刘长安[2] 龚建平[2] 

机构地区：[1]遂宁市中心医院肝胆外科,四川遂宁629000 [2]重庆医科大学第二临床学院肝胆外科,重庆400010

出　　处：《实用肿瘤杂志》2009年第5期453-457,共5页Journal of Practical Oncology

基　　金：国家自然科学基金资助项目(30400424)

摘　　要：目的观察环氧化酶-2(COX-2)、P糖蛋白(P-gp)在肝细胞肝癌(HCC)中的表达并分析两者的相关性。方法应用免疫组化S-P法、逆转录多聚酶链反应法(RT-PCR)检测重庆医科大学附属第二医院2005年10月～2007年6月手术切除经病理证实的52例HCC标本、癌旁组织及10例正常肝组织中COX-2和P-gp及其基因的表达情况。结果COX-2/COX-2 mRNA、P-gp/MDR1 mRNA在肝细胞肝癌组织中的表达明显上调,与其在正常肝组织、癌旁组织中的表达比较差异有统计学意义(P<0.01)。且COX-2、P-gp在肝细胞肝癌中的阳性表达具有明显相关性,相关系数r=0.563(P<0.01)。结论(1)COX-2高表达参与了肝癌的发生、发展。(2)COX-2与P-gp在肝细胞肝癌中的表达密切相关,COX-2可能干预P-gp的表达从而参与肝癌的多药耐药过程。Objective To investigaste the expression of COX-2 and P-gp in HCC tissue and to analyze the correlation between the COX-2 and P-gp expression. Methods Fifty-two samples of excisional specimen which were confirmed as HCC through pathological section in Second Affiliated Hospital of Chongqing University of Medical Sciences from October,2005 to June,2007 were selected to study.With the method of immunohistochemistry and RT-PCR,the expression levels of COX-2,COX-2mRNA and P-gp,MDR1mRNA in the 52 specimens of HCC,homologous latero-HCC tissue and 10 normal liver tissue were determined,respectively. Results COX-2/COX-2mRNA,P-gp/MDR1mRNA expressed obviously higher than those in latero-HCC and normal tissues（P〈0.01）.It showed significant correlation with P-gp and COX-2.The correlation coefficient was r=0.563 （P〈0.01）. Conclusions （1）COX-2 overexpression takes part in the process of the birth,growth of hepatic cancer.（2） COX-2 and P-gp are tightly related in the expression of HCC.COX-2 intervenes in the expression of P-gp.Accordingly COX-2 takes part in the procedures of MDR of HCC.

关 键 词：肝肿瘤 前列腺素内过氧化物合酶 基因 MDR P-糖蛋白 药物疗法 

分 类 号：R735.7[医药卫生—肿瘤]