靛玉红甲肟对HT-29细胞STAT3和Bcl-2/Bax表达的影响  

Effects of Indirubin-3'-monoxime on expression of STAT3 and Bcl-2/Bax in HT-29 cells

作　　者：陈小兵[1,2] 曹新广[2] 张军辉[3] 董文杰[4] 罗素霞[2] 

机构地区：[1]郑州大学第一附属医院肿瘤内科,河南郑州450052 [2]河南省肿瘤医院肿瘤内科,河南郑州450008 [3]郑州大学第一附属医院耳鼻咽喉科,河南郑州450052 [4]上海交通大学附属瑞金医院消化科,上海200025

出　　处：《实用肿瘤杂志》2009年第5期458-461,共4页Journal of Practical Oncology

基　　金：河南省医药卫生重大攻关项目;卫生部科研基金资助项目(WKJ2007-2-026)

摘　　要：目的检测靛玉红甲肟(Indirubin-3’-monoxime,IRO)作用前后人结肠癌HT-29细胞转录活化因子3(STAT3)和凋亡调节基因Bcl-2/Bax表达变化,探讨IRO抑制HT-29细胞增殖的机制。方法MTT法检测不同浓度、不同作用时间IRO对HT-29细胞增殖活性的影响。RT-PCR法检测10μmol/L的IRO作用不同时间对HT-29细胞STAT3、Bcl-2和BaxmRNA表达的影响。结果IRO对HT-29细胞生长具有明显的增殖抑制作用,且表现为剂量依赖性和时间依赖性(P<0.01)。RT-PCR检测发现,以10μmol/L的IRO处理HT-29细胞后,STAT3和Bcl-2表达显著下降,而Bax表达上升,不同时间组间差异有统计学意义(P<0.01)。结论IRO具有抑制人结肠癌HT-29细胞增殖的作用,其机制与下调STAT3、Bcl-2表达和升高Bax表达有关。Objective To detect STAT3 and Bcl-2/Bax expression changes in human colon carcinoma HT-29 cells before and after treated with Indirubin-3＇-monoxime（IRO） and to explore its role in HT-29 proliferation inhibition. Methods Methabenzthiazuron（MTT） was used to observe the proliferation of human colon carcinoma HT-29 cells treated with various concentrations of IRO at different time.RT-PCR was used to measure the expression rates of STAT3,Bcl-2 and Bax mRNA in the HT-29 cells treated with IRO at 10 μmol/L for different time. Results IRO inhibited growth of HT-29 cells in a dose-dependent and time-dependent manner（P〈0.01）.The expressions of STAT3 and Bcl-2 mRNA of HT-29 cells were decreased and the expression of Bax was significantly inecreased.There were significant differences between different time groups （P〈0.01）. Conclusions IRO can inhibit the proliferation of human colon carcinoma HT-29 cells,its mechanism is related to reduced STAT3 and Bcl-2 expression and increased Bax expression.

关 键 词：结肠肿瘤 靛玉红甲肟 肿瘤细胞 培养的 基因 BCL-2 基因 Bax 细胞凋亡 

分 类 号：R735.3[医药卫生—肿瘤]