高效氯氰菊酯降解菌JCN13的分离鉴定及降解特性研究  

Isolation,Identification and Degradation Characteristics of Beta-cypermethrin Degrading Strain JCN13

作　　者：张琛[1] 王圣惠[2] 闫艳春[2] 

机构地区：[1]山东农业大学生命科学学院,泰安271018 [2]中国农业科学院研究生院,北京100081

出　　处：《生物技术通报》2009年第11期174-177,共4页Biotechnology Bulletin

基　　金：国家高科技研究发展计划"863计划"(2008AA10Z402;2006AA10Z402)

摘　　要：从生产高效氯氰菊酯的农药厂污水曝气池中,分离到一株能降解高效氯氰菊酯并以之为唯一碳源进行生长的细菌JCN13。经生理生化试验和16S rDNA分析,鉴定菌株JCN13为沙雷菌属(Serratia sp.)。气相色谱检测,菌株JCN13在4d内对100mg/L高效氯氰菊酯的降解率为89%,8d内基本降解完全。经气质联用检测,发现高效氯氰菊酯在被菌株JCN13降解的过程中存在异构体的转化。Strain JCN13 which was isolated from polluted wastes of pesticide plant,could degrade beta-cypermethrin and utilize it as the sole carbon resource for growth. Based on the results of physiological-biochemical properties and phylogenetic similarity of 16S rDNA gene sequences,strain JCN13 was identified as Serratia sp. After being cultivated for 4 days,the strain could degrad 89% betacypermethrin and complete degradation was observed in 8 days. The GC-MS analysis showed that certain isomerization took place during the beta-cypermethrin degradation process by strain JCN13.

关 键 词：高效氯氰菊酯 沙雷菌属 生物降解 异构化 

分 类 号：X172[环境科学与工程—环境科学]