豆天蛾核型多角体病毒光解酶变异基因序列的解析  

Sequence Analysis of Variant Photolyase Gene of Clanis bilineata Nucleopolyhedrovirus

作　　者：朱姗颖[1,2] 易建平 何华纲[2] 董英[2] 王文兵[1] 

机构地区：[1]江苏大学生命科学研究院,江苏镇江212013 [2]江苏大学食品与生物工程学院,江苏镇江212013 [3]上海市出入境检验检疫局,上海200135

出　　处：《江苏农业学报》2009年第5期980-985,共6页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省六大人才高峰基金项目(2005-农-21);江苏省研究生培养创新工程项目(2006);江苏省高校自然科学指导性计划项目(06KJD180043)

摘　　要：对新近发现的豆天蛾核型多角体病毒(CbNPV)进行分离纯化,提取其基因组DNA,用Shotgun法构建了DNA片段的文库,并进行了全基因组测序。根据序列分析的结果,发现了一段长为1 694 bp的光解酶类似基因序列,但与已报道的杆状病毒光解酶基因序列相对比,发生了一个碱基的缺失,从而使其分裂成2个小的读码框:Cbphr1-和Cbphr2-。结构域分析结果表明,所有已知DNA光解酶都具有的CPD-DNA光解酶结构域和FAD-结合结构域在CbNPV中也存在。Polyhedron particles of Clanis bilineata tiainglauica nucleopolyhedrovirus （CbNPV）, a novel baculovirus, was isolated and its genomic DNA was extracted. A DNA fragment library of the virus was constructed by shotgun method, and the complete genome sequence of CbNPV was determined. Sequence analysis revealed the presence of a photolyaselike gene with 1 694 bp in length. Compared with photolyase genes among other baculoviruses, it was interesting that there was a 1 bp deletion mutation. This deletion disrupted the sequence into two small ORFs：Cbphr-1 and Cbphr-2. Both the DNA photolyase and FAD-binding domains are conserved in the CbNPV.

关 键 词：豆天蛾核型多角体病毒 光解酶 序列分析 

分 类 号：Q523.8[生物学—生物化学]