MTT法检测多种肿瘤细胞对硒酸酯多糖的敏感性研究  

Detection the Sensitivity of Several Cancer Cell Lines to Kappa Selenocarrageenan by MTT Assay

作　　者：邓丹[1,2] 凌娜[1,2] 季宇彬[1,2] 

机构地区：[1]哈尔滨商业大学生命科学与环境科学研究中心,黑龙江哈尔滨150076 [2]国家教育部抗肿瘤天然药物工程研究中心,黑龙江哈尔滨150076

出　　处：《亚太传统医药》2009年第10期16-17,共2页Asia-Pacific Traditional Medicine

摘　　要：目的:采用MTT法检测多种肿瘤细胞对硒酸酯多糖(KSC)的敏感性。方法:以30、60、120mg/L3种不同浓度的KSC分别处理HepG-2、Bel-7402、MCF-7、SGC-7901、A5495种肿瘤细胞,采用MTT法检测KSC对5种肿瘤细胞的抑制率。结果:KSC对多种肿瘤细胞都有明显的抑制增殖作用,KSC120mg/L作用72h后,对上述5种细胞株的抑制率分别为69.31%、62.24%、53.29%、52.03%、33.62%。结论:KSC对多种肿瘤细胞都有抑制作用,尤其是对肝癌细胞的抑制作用最强,具有治疗肝癌潜在的应用价值。Objective ：To detect the sensitivity of several cancer cell lines to KappaSelenocarrageenan by M＇IT assay.Methods：Five cancer cell lines included HepG-2,Bel-7402,MCF-7,SGC-7901 and A549 were incubated with Kappa-Selenocarrageenan of various concent ration （30mg/L,60mg/L, 120mg/L）.The inhibition rate was examined by MTT test assay.Results：KSC had a significant killing effect on human cancer ceils in vitro.72 hours latter, the inhibition rates of HepG-2,Bel-7402,MCF-7,SGC-7901and A549 with 120 mg/L KSC separately were 69.31% ,62.24% ,53.29% ,52.03% ,33.62%.Conclusion：KSC has a wide range of inhibition of tumor cells, especially for the strongest inhibition of hepatic carcinoma cells.It might have a promising prospect in the treatment for hepatic carcinoma.

关 键 词：MTT法 硒酸酯多糖(KSC) 肿瘤细胞 敏感性 

分 类 号：R-33[医药卫生]